CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018733
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nicastrin 
Protein Synonyms/Alias
  
Gene Name
 NCSTN 
Gene Synonyms/Alias
 KIAA0253; UNQ1874/PRO4317 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
110FTRDLMEKLKGRTSRubiquitination[1]
127GLAVSLTKPSPASGFubiquitination[1]
349RMVYDMEKGKFPVQLubiquitination[1]
351VYDMEKGKFPVQLENubiquitination[1, 2, 3]
403DLLATLEKSGAGVPAubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. 
Sequence Annotation
 CARBOHYD 45 45 N-linked (GlcNAc...).
 CARBOHYD 55 55 N-linked (GlcNAc...) (Potential).
 CARBOHYD 187 187 N-linked (GlcNAc...).
 CARBOHYD 200 200 N-linked (GlcNAc...) (Potential).
 CARBOHYD 204 204 N-linked (GlcNAc...) (Potential).
 CARBOHYD 264 264 N-linked (GlcNAc...) (Potential).
 CARBOHYD 387 387 N-linked (GlcNAc...).
 CARBOHYD 417 417 N-linked (GlcNAc...) (Potential).
 CARBOHYD 435 435 N-linked (GlcNAc...) (Potential).
 CARBOHYD 464 464 N-linked (GlcNAc...) (Potential).
 CARBOHYD 506 506 N-linked (GlcNAc...) (Potential).
 CARBOHYD 530 530 N-linked (GlcNAc...) (Potential).
 CARBOHYD 562 562 N-linked (GlcNAc...) (Potential).
 CARBOHYD 573 573 N-linked (GlcNAc...) (Potential).
 CARBOHYD 580 580 N-linked (GlcNAc...) (Potential).
 CARBOHYD 612 612 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Complete proteome; Disease mutation; Glycoprotein; Membrane; Notch signaling pathway; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 709 AA 
Protein Sequence
MATAGGGSGA DPGSRGLLRL LSFCVLLAGL CRGNSVERKI YIPLNKTAPC VRLLNATHQI 60
GCQSSISGDT GVIHVVEKEE DLQWVLTDGP NPPYMVLLES KHFTRDLMEK LKGRTSRIAG 120
LAVSLTKPSP ASGFSPSVQC PNDGFGVYSN SYGPEFAHCR EIQWNSLGNG LAYEDFSFPI 180
FLLEDENETK VIKQCYQDHN LSQNGSAPTF PLCAMQLFSH MHAVISTATC MRRSSIQSTF 240
SINPEIVCDP LSDYNVWSML KPINTTGTLK PDDRVVVAAT RLDSRSFFWN VAPGAESAVA 300
SFVTQLAAAE ALQKAPDVTT LPRNVMFVFF QGETFDYIGS SRMVYDMEKG KFPVQLENVD 360
SFVELGQVAL RTSLELWMHT DPVSQKNESV RNQVEDLLAT LEKSGAGVPA VILRRPNQSQ 420
PLPPSSLQRF LRARNISGVV LADHSGAFHN KYYQSIYDTA ENINVSYPEW LSPEEDLNFV 480
TDTAKALADV ATVLGRALYE LAGGTNFSDT VQADPQTVTR LLYGFLIKAN NSWFQSILRQ 540
DLRSYLGDGP LQHYIAVSSP TNTTYVVQYA LANLTGTVVN LTREQCQDPS KVPSENKDLY 600
EYSWVQGPLH SNETDRLPRC VRSTARLARA LSPAFELSQW SSTEYSTWTE SRWKDIRARI 660
FLIASKELEL ITLTVGFGIL IFSLIVTYCI NAKADVLFIA PREPGAVSY 709 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0005887; C:integral to plasma membrane; ISS:UniProtKB.
 GO:0005765; C:lysosomal membrane; IEA:Compara.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0004175; F:endopeptidase activity; IEA:Compara.
 GO:0042987; P:amyloid precursor protein catabolic process; TAS:HGNC.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0050435; P:beta-amyloid metabolic process; IEA:Compara.
 GO:0050673; P:epithelial cell proliferation; IEA:Compara.
 GO:0006509; P:membrane protein ectodomain proteolysis; ISS:UniProtKB.
 GO:0031293; P:membrane protein intracellular domain proteolysis; TAS:Reactome.
 GO:0002262; P:myeloid cell homeostasis; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0007220; P:Notch receptor processing; TAS:HGNC.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0043085; P:positive regulation of catalytic activity; ISS:UniProtKB.
 GO:0016485; P:protein processing; ISS:UniProtKB.
 GO:0042098; P:T cell proliferation; IEA:Compara. 
Interpro
 IPR008710; Nicastrin. 
Pfam
 PF05450; Nicastrin 
SMART
  
PROSITE
  
PRINTS