UniProt Accession

 GRHPR_MOUSE; Q91Z53 

Genbank Protein ID

 AY113690; BC010194 

Genbank Nucleotide ID

 AAM52985.1; AAH10194.1 

Protein Name

 Glyoxylate reductase/hydroxypyruvate reductase 

Protein Synonyms/Alias

  

Gene Name

 Grhpr 

Gene Synonyms/Alias

 Glxr 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
65	RLSDRVDKKLLDAAG	acetylation	[1]
233	CSKDFFQKMKNTAIF	acetylation	[1]
300	HIGSATYKTRNTMSL	ubiquitination	[2]
327	EAMPSELKL******	acetylation	[3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate (By similarity). 

Sequence Annotation

 NP_BIND 162 164 NADP (By similarity).
 NP_BIND 185 188 NADP (By similarity).
 REGION 83 84 Substrate binding (By similarity).
 REGION 293 296 Substrate binding (By similarity).
 ACT_SITE 293 293 Proton donor (By similarity).
 BINDING 217 217 NADP (By similarity).
 BINDING 243 243 NADP; via carbonyl oxygen (By
 BINDING 245 245 Substrate (By similarity).
 BINDING 269 269 Substrate (By similarity).
 BINDING 295 295 NADP; via amide nitrogen (By similarity).  

Keyword

 Complete proteome; Direct protein sequencing; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 328 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0008465; F:glycerate dehydrogenase activity; IEA:Compara.
 GO:0030267; F:glyoxylate reductase (NADP) activity; IEA:EC.
 GO:0016618; F:hydroxypyruvate reductase activity; IEA:EC.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0070402; F:NADPH binding; IEA:Compara.
 GO:0007588; P:excretion; IEA:Compara.
 GO:0051259; P:protein oligomerization; IEA:Compara. 

Interpro

 IPR006139; D-isomer_2_OHA_DH_cat_dom.
 IPR006140; D-isomer_2_OHA_DH_NAD-bd.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00389; 2-Hacid_dh
 PF02826; 2-Hacid_dh_C 

SMART

  

PROSITE

 PS00065; D_2_HYDROXYACID_DH_1
 PS00670; D_2_HYDROXYACID_DH_2
 PS00671; D_2_HYDROXYACID_DH_3 

PRINTS