UniProt Accession

 PEF1_HUMAN; Q9UBV8 

Genbank Protein ID

 AB026628; AB018357; AY359011; AK001420; CR542139; BC002773; BC012561 

Genbank Nucleotide ID

 BAA85163.1; BAA84922.1; AAQ89370.1; BAA91680.1; CAG46936.1; AAH02773.1; AAH12561.1 

Protein Name

 Peflin 

Protein Synonyms/Alias

 PEF protein with a long N-terminal hydrophobic domain; Penta-EF hand domain-containing protein 1 

Gene Name

 PEF1 

Gene Synonyms/Alias

 ABP32; UNQ1845/PRO3573 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
260	LTEAFREKDTAVQGN	ubiquitination	[1, 2, 3]

Reference

 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

  

Sequence Annotation

 REPEAT 21 29 1.
 REPEAT 31 39 2.
 REPEAT 41 49 3.
 REPEAT 50 58 4.
 REPEAT 59 67 5.
 REPEAT 76 84 6.
 REPEAT 85 92 7.
 REPEAT 93 100 8.
 REPEAT 101 109 9.
 DOMAIN 114 149 EF-hand 1.
 DOMAIN 155 183 EF-hand 2.
 DOMAIN 181 216 EF-hand 3.
 DOMAIN 217 253 EF-hand 4.
 DOMAIN 254 283 EF-hand 5.
 REGION 21 109 9 X 9 AA approximate tandem repeat of
 REGION 204 284 Required for interaction with PDCD6.  

Keyword

 Calcium; Complete proteome; Cytoplasm; Membrane; Metal-binding; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 284 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; TAS:ProtInc.
 GO:0051592; P:response to calcium ion; IPI:UniProtKB. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 

Pfam

 PF13499; EF_hand_5 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS