CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008918
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gamma-glutamyl phosphate reductase 
Protein Synonyms/Alias
 GPR; Glutamate-5-semialdehyde dehydrogenase; GSA dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase 
Gene Name
 PRO2 
Gene Synonyms/Alias
 YOR323C; O6155 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
9SSSQQIAKNARKAGNacetylation[1]
32GRSDILYKIHDALKAacetylation[1]
49HAIEEANKIDLAVAKacetylation[1]
66GLADSLLKRLDLFKGacetylation[1]
72LKRLDLFKGDKFEVMacetylation[1]
301LKTAYFDKLNELGKLacetylation[1]
301LKTAYFDKLNELGKLubiquitination[2]
370ENKANAEKFMKGVDSubiquitination[2]
447GNKAFVHKDLDIKTVacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. 
Sequence Annotation
  
Keyword
 3D-structure; Amino-acid biosynthesis; Complete proteome; NADP; Oxidoreductase; Proline biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 456 AA 
Protein Sequence
MSSSQQIAKN ARKAGNILKT ISNEGRSDIL YKIHDALKAN AHAIEEANKI DLAVAKETGL 60
ADSLLKRLDL FKGDKFEVML QGIKDVAELE DPVGKVKMAR ELDDGLTLYQ VTAPVGVLLV 120
IFESRPEVIA NITALSIKSG NAAILKGGKE SVNTFREMAK IVNDTIAQFQ SETGVPVGSV 180
QLIETRQDVS DLLDQDEYID LVVPRGSNAL VRKIKDTTKI PVLGHADGIC SIYLDEDADL 240
IKAKRISLDA KTNYPAGCNA METLLINPKF SKWWEVLENL TLEGGVTIHA TKDLKTAYFD 300
KLNELGKLTE AIQCKTVDAD EEQDFDKEFL SLDLAAKFVT STESAIQHIN THSSRHTDAI 360
VTENKANAEK FMKGVDSSGV YWNASTRFAD GFRYGFGAEV GISTSKIHAR GPVGLDGLVS 420
YQYQIRGDGQ VASDYLGAGG NKAFVHKDLD IKTVTL 456 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0004350; F:glutamate-5-semialdehyde dehydrogenase activity; IDA:SGD.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006561; P:proline biosynthetic process; IDA:SGD. 
Interpro
 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom.
 IPR000965; G-glutamylP_reductase.
 IPR020593; G-glutamylP_reductase_CS.
 IPR012134; Glu-5-SA_DH. 
Pfam
 PF00171; Aldedh 
SMART
  
PROSITE
 PS01223; PROA 
PRINTS