CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014279
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin subunit alpha-5 
Protein Synonyms/Alias
 Importin alpha-S1; Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1; RAG cohort protein 2; SRP1-beta 
Gene Name
 Kpna1 
Gene Synonyms/Alias
 Rch2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
136ARFVEFLKRKENCTLubiquitination[1]
398TAEFRTRKEAAWAITubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. 
Sequence Annotation
 DOMAIN 1 57 IBB.
 REPEAT 77 117 ARM 1; truncated.
 REPEAT 118 161 ARM 2.
 REPEAT 162 206 ARM 3.
 REPEAT 207 245 ARM 4.
 REPEAT 246 290 ARM 5.
 REPEAT 291 330 ARM 6.
 REPEAT 331 372 ARM 7.
 REPEAT 373 412 ARM 8.
 REPEAT 413 457 ARM 9.
 REPEAT 460 504 ARM 10; atypical.
 REGION 149 241 NLS binding site (major) (By similarity).
 REGION 245 437 Binding to RAG1 (By similarity).
 REGION 318 406 NLS binding site (minor) (By similarity).
 MOTIF 42 51 Nuclear localization signal (By
 MOD_RES 3 3 Phosphothreonine.  
Keyword
 Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 538 AA 
Protein Sequence
MSTPGKENFR LKSYKNKSLN PDEMRRRREE EGLQLRKQKR EEQLFKRRNV ATAEEETEEE 60
VMSDGGFHEA QINNMEMAPG GVITSDMTDM IFSNSPEQQL SATQKFRKLL SKEPNPPIDE 120
VINTPGVVAR FVEFLKRKEN CTLQFESAWV LTNIASGNSL QTRNVIQAGA VPIFIELLSS 180
EFEDVQEQAV WALGNIAGDS TMCRDYVLNC NILPPLLQLF SKQNRLTMTR NAVWALSNLC 240
RGKSPPPEFA KVSPCLNVLS WLLFVSDTDV LADACWALSY LSDGPNDKIQ AVIDAGVCRR 300
LVELLMHNDY KVVSPALRAV GNIVTGDDIQ TQVILNCSAL QSLLHLLSSP KESIKKEACW 360
TISNITAGNR AQIQTVIDAN MFPALISILQ TAEFRTRKEA AWAITNATSG GSAEQIKYLV 420
ELGCIKPLCD LLTVMDAKIV QVALNGLENI LRLGEQEAKR NGSGINPYCA LIEEAYGLDK 480
IEFLQSHENQ EIYQKAFDLI EHYFGTEDED SSIAPQVDLS QQQYIFQQCE APMEGFQL 538 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030425; C:dendrite; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0042307; P:positive regulation of protein import into nucleus; ISS:UniProtKB.
 GO:0006606; P:protein import into nucleus; IEA:InterPro. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR002652; Importin-a_IBB.
 IPR024931; Importing_su_alpha. 
Pfam
 PF00514; Arm
 PF01749; IBB 
SMART
 SM00185; ARM 
PROSITE
 PS50176; ARM_REPEAT
 PS51214; IBB 
PRINTS