CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017635
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 280C 
Protein Synonyms/Alias
 Suppressor of hairy wing homolog 3; Zinc finger protein 633 
Gene Name
 ZNF280C 
Gene Synonyms/Alias
 SUHW3; ZNF633 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14FQPKNISKMAELFMEubiquitination[1]
32EELEPWQKKVEETQDubiquitination[1]
33ELEPWQKKVEETQDEubiquitination[1]
75SSSSKGIKSEPHSPGsumoylation[2]
241DYLRACPKCNVQFNLubiquitination[1]
273KFLGVIVKSERPCDEsumoylation[2]
610NKMSLALKNIRCRRGubiquitination[3, 4]
689GITLVCLKCDFLADSubiquitination[1, 3, 4, 5, 6, 7, 8]
737PTTGCSLK*******ubiquitination[8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 May function as a transcription factor. 
Sequence Annotation
 ZN_FING 316 338 C2H2-type 1.
 ZN_FING 353 376 C2H2-type 2.
 ZN_FING 383 406 C2H2-type 3.
 ZN_FING 413 436 C2H2-type 4.
 ZN_FING 470 492 C2H2-type 5.
 MOD_RES 80 80 Phosphoserine.
 MOD_RES 227 227 Phosphoserine.
 MOD_RES 540 540 Phosphothreonine.  
Keyword
 Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 737 AA 
Protein Sequence
MDDDKPFQPK NISKMAELFM ECEEEELEPW QKKVEETQDE DDDELIFVGE ISSSKPAISN 60
ILNRGHSSSS SKGIKSEPHS PGIPEIFRTA SQRCRDPPSN PVAASPRFHL VSKSSQSSVT 120
VENASKPDFT KNSQVGSDNS SILLFDSTQE SLPPSQDIPA IFREGMKNTS YVLKHPSTSK 180
VNSVTPKKPK TSEDVPQINP STSLPLIGSP PVTSSQVMLS KGTNTSSPYD AGADYLRACP 240
KCNVQFNLLD PLKYHMKHCC PDMITKFLGV IVKSERPCDE DKTDSETGKL IMLVNEFYYG 300
RHEGVTEKEP KTYTTFKCFS CSKVLKNNIR FMNHMKHHLE LEKQNNESWE NHTTCQHCYR 360
QYPTPFQLQC HIESTHTPHE FSTICKICEL SFETEHILLQ HMKDTHKPGE MPYVCQVCQF 420
RSSTFSDVEA HFRAAHENTK NLLCPFCLKV SKMATPYMNH YMKHQKKGVH RCPKCRLQFL 480
TSKEKAEHKA QHRTFIKPKE LEGLPPGAKV TIRASLGPLQ SKLPTAPFGC APGTSFLQVT 540
PPTSQNTTAR NPRKSNASRS KTSKLHATTS TASKVNTSKP RGRIAKSKAK PSYKQKRQRN 600
RKNKMSLALK NIRCRRGIHK CIECHSKIKD FASHFSIYIH CSFCKYNTNC NKAFVNHMMS 660
SHSNHPGKRF CIFKKHSGTL RGITLVCLKC DFLADSSGLD RMAKHLSQRK THTCQVIIEN 720
VSKSTSTSEP TTGCSLK 737 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR025243; DUF4195.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
 PF13836; DUF4195 
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS