CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001110
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NEDD4-binding protein 1 
Protein Synonyms/Alias
 N4BP1 
Gene Name
 N4BP1 
Gene Synonyms/Alias
 KIAA0615 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16EFTAPAEKAELLEQSubiquitination[1]
70HSAKEYIKGICEPELubiquitination[1]
239LQEEARNKAGTPVSEubiquitination[1]
297SEERHTKKQFSLENVubiquitination[1, 2, 3]
366YSQEIVEKVIKVYGPubiquitination[4]
369EIVEKVIKVYGPSTEubiquitination[5]
408TVYPETNKTKNKGVYubiquitination[1]
412ETNKTKNKGVYSSTNubiquitination[1, 5]
441TQQNMVEKFSQLPFKubiquitination[2, 3]
601QRFRDTLKIPYKLELubiquitination[1, 2, 3]
773RLEEFLQKEVCLRDMubiquitination[1, 6, 7]
863ELREALLKIFPDSEQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity). 
Sequence Annotation
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 258 258 Phosphoserine.
 MOD_RES 300 300 Phosphoserine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 896 AA 
Protein Sequence
MAARAVLDEF TAPAEKAELL EQSRGRIEGL FGVSLAVLGA LGAEEPLPAR IWLQLCGAQE 60
AVHSAKEYIK GICEPELEER ECYPKDMHCI FVGAESLFLK SLIQDTCADL CILDIGLLGI 120
RGSAEAVVMA RSHIQQFVKL FENKENLPSS QKESEVKREF KQFVEAHADN YTMDLLILPT 180
SLKKELLTLT QGEENLFETG DDEVIEMRDS QQTEFTQNAA TGLNISRDET VLQEEARNKA 240
GTPVSELTKQ MDTVLSSSPD VLFDPINGLT PDEEALSNER ICQKRRFSDS EERHTKKQFS 300
LENVQEGEIL HDAKTLAGNV IADLSDSSAD SENLSPDIKE TTEEMEYNIL VNFFKTMGYS 360
QEIVEKVIKV YGPSTEPLLL LEEIEKENKR FQEDREFSAG TVYPETNKTK NKGVYSSTNE 420
LTTDSTPKKT QAHTQQNMVE KFSQLPFKVE AKPCTSNCRI NTFRTVPIEQ KHEVWGSNQN 480
YICNTDPETD GLSPSVASPS PKEVNFVSRG ASSHQPRVPL FPENGLHQQP EPLLPNNMKS 540
ACEKRLGCCS SPHSKPNCST LSPPMPLPQL LPSVTDARSA GPSDHIDSSV TGVQRFRDTL 600
KIPYKLELKN EPGRTDLKHI VIDGSNVAIT HGLKKFFSCR GIAIAVEYFW KLGNRNITVF 660
VPQWRTRRDP NVTEQHFLTQ LQELGILSLT PARMVFGERI ASHDDRFLLH LADKTGGIIV 720
TNDNFREFVN ESVSWREIIT KRLLQYTFVG DIFMVPDDPL GRSGPRLEEF LQKEVCLRDM 780
QPLLSALPNV GMFDPSFRVP GTQAASTSHQ PPTRIQGAPS SHWLPQQPHF PLLPALPSLQ 840
QNLPMPAQRS SAETNELREA LLKIFPDSEQ RLKIDQILVA HPYMKDLNAL SAMVLD 896 
Gene Ontology
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0016605; C:PML body; ISS:UniProtKB.
 GO:0034644; P:cellular response to UV; IMP:BHF-UCL.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB. 
Interpro
 IPR021869; RNase_Zc3h12. 
Pfam
 PF11977; RNase_Zc3h12a 
SMART
  
PROSITE
  
PRINTS