UniProt Accession

 ARAF_HUMAN; P10398; P07557; Q5H9B2; Q5H9B3 

Genbank Protein ID

 X04790; L24038; U01337; AB158254; BT019864; AL542736; Z84466; Z84466; BC002466; M13829 

Genbank Nucleotide ID

 CAA28476.1; AAA65219.1; AAB03517.1; BAE66645.1; AAV38667.1; CAI42468.1; CAI42469.1; AAH02466.1; AAB08754.1 

Protein Name

 Serine/threonine-protein kinase A-Raf 

Protein Synonyms/Alias

 Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks 

Gene Name

 ARAF 

Gene Synonyms/Alias

 ARAF1; PKS; PKS2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
423	GMDYLHAKNIIHRDL	ubiquitination	[1]
454	DFGLATVKTRWSGAQ	ubiquitination	[1]
551	RLLSDCLKFQREERP	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. 

Sequence Annotation

 DOMAIN 19 91 RBD.
 DOMAIN 310 570 Protein kinase.
 ZN_FING 98 144 Phorbol-ester/DAG-type.
 NP_BIND 316 324 ATP (By similarity).
 ACT_SITE 429 429 Proton acceptor (By similarity).
 METAL 99 99 Zinc 1 (By similarity).
 METAL 112 112 Zinc 2 (By similarity).
 METAL 115 115 Zinc 2 (By similarity).
 METAL 125 125 Zinc 1 (By similarity).
 METAL 128 128 Zinc 1 (By similarity).
 METAL 133 133 Zinc 2 (By similarity).
 METAL 136 136 Zinc 2 (By similarity).
 METAL 144 144 Zinc 1 (By similarity).
 BINDING 336 336 ATP (By similarity).
 MOD_RES 186 186 Phosphoserine.
 MOD_RES 257 257 Phosphoserine.
 MOD_RES 318 318 Phosphothreonine.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 606 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0004709; F:MAP kinase kinase kinase activity; IEA:Compara.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IDA:UniProtKB. 

Interpro

 IPR020454; DAG/PE-bd.
 IPR011009; Kinase-like_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR003116; Raf-like_ras-bd.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00130; C1_1
 PF07714; Pkinase_Tyr
 PF02196; RBD 

SMART

 SM00109; C1
 SM00455; RBD 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50898; RBD
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 

PRINTS

 PR00008; DAGPEDOMAIN.