CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022824
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 C-type mannose receptor 2 
Protein Synonyms/Alias
 C-type lectin domain family 13 member E; Endocytic receptor 180; Macrophage mannose receptor 2; Urokinase-type plasminogen activator receptor-associated protein; UPAR-associated protein; Urokinase receptor-associated protein; CD280 
Gene Name
 MRC2 
Gene Synonyms/Alias
 CLEC13E; ENDO180; KIAA0709; UPARAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
957CKRSNVTKETQPPDLubiquitination[1]
1142GTFRLLQKPLRWHDAsumoylation[2]
1142GTFRLLQKPLRWHDAubiquitination[3]
1394ITMGVVCKLPRAEQSubiquitination[4]
1465SPTEATEKNILVSDMubiquitination[4, 5]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] In vivo identification of sumoylation sites by a signature tag and cysteine-targeted affinity purification.
 Blomster HA, Imanishi SY, Siimes J, Kastu J, Morrice NA, Eriksson JE, Sistonen L.
 J Biol Chem. 2010 Jun 18;285(25):19324-9. [PMID: 20388717]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs). 
Sequence Annotation
 DOMAIN 41 167 Ricin B-type lectin.
 DOMAIN 182 230 Fibronectin type-II.
 DOMAIN 244 360 C-type lectin 1.
 DOMAIN 389 505 C-type lectin 2.
 DOMAIN 528 644 C-type lectin 3.
 DOMAIN 678 809 C-type lectin 4.
 DOMAIN 832 951 C-type lectin 5.
 DOMAIN 979 1107 C-type lectin 6.
 DOMAIN 1132 1243 C-type lectin 7.
 DOMAIN 1273 1393 C-type lectin 8.
 CARBOHYD 69 69 N-linked (GlcNAc...).
 CARBOHYD 140 140 N-linked (GlcNAc...) (Potential).
 CARBOHYD 364 364 N-linked (GlcNAc...) (Potential).
 CARBOHYD 588 588 N-linked (GlcNAc...) (Potential).
 CARBOHYD 954 954 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1029 1029 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1350 1350 N-linked (GlcNAc...) (Potential).
 DISULFID 54 68 By similarity.
 DISULFID 93 112 By similarity.
 DISULFID 187 213 By similarity.
 DISULFID 201 228 By similarity.
 DISULFID 266 359 By similarity.
 DISULFID 335 351 By similarity.
 DISULFID 410 504 By similarity.
 DISULFID 481 496 By similarity.
 DISULFID 618 635 By similarity.
 DISULFID 704 808 By similarity.
 DISULFID 785 800 By similarity.
 DISULFID 853 950 By similarity.
 DISULFID 927 942 By similarity.
 DISULFID 1078 1098 By similarity.
 DISULFID 1220 1234 By similarity.
 DISULFID 1369 1384 By similarity.
 CROSSLNK 1142 1142 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Calcium; Complete proteome; Direct protein sequencing; Disulfide bond; Endocytosis; Glycoprotein; Isopeptide bond; Lectin; Membrane; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1479 AA 
Protein Sequence
MGPGRPAPAP WPRHLLRCVL LLGCLHLGRP GAPGDAALPE PNVFLIFSHG LQGCLEAQGG 60
QVRVTPACNT SLPAQRWKWV SRNRLFNLGT MQCLGTGWPG TNTTASLGMY ECDREALNLR 120
WHCRTLGDQL SLLLGARTSN ISKPGTLERG DQTRSGQWRI YGSEEDLCAL PYHEVYTIQG 180
NSHGKPCTIP FKYDNQWFHG CTSTGREDGH LWCATTQDYG KDERWGFCPI KSNDCETFWD 240
KDQLTDSCYQ FNFQSTLSWR EAWASCEQQG ADLLSITEIH EQTYINGLLT GYSSTLWIGL 300
NDLDTSGGWQ WSDNSPLKYL NWESDQPDNP SEENCGVIRT ESSGGWQNRD CSIALPYVCK 360
KKPNATAEPT PPDRWANVKV ECEPSWQPFQ GHCYRLQAEK RSWQESKKAC LRGGGDLVSI 420
HSMAELEFIT KQIKQEVEEL WIGLNDLKLQ MNFEWSDGSL VSFTHWHPFE PNNFRDSLED 480
CVTIWGPEGR WNDSPCNQSL PSICKKAGQL SQGAAEEDHG CRKGWTWHSP SCYWLGEDQV 540
TYSEARRLCT DHGSQLVTIT NRFEQAFVSS LIYNWEGEYF WTALQDLNST GSFFWLSGDE 600
VMYTHWNRDQ PGYSRGGCVA LATGSAMGLW EVKNCTSFRA RYICRQSLGT PVTPELPGPD 660
PTPSLTGSCP QGWASDTKLR YCYKVFSSER LQDKKSWVQA QGACQELGAQ LLSLASYEEE 720
HFVANMLNKI FGESEPEIHE QHWFWIGLNR RDPRGGQSWR WSDGVGFSYH NFDRSRHDDD 780
DIRGCAVLDL ASLQWVAMQC DTQLDWICKI PRGTDVREPD DSPQGRREWL RFQEAEYKFF 840
EHHSTWAQAQ RICTWFQAEL TSVHSQAELD FLSHNLQKFS RAQEQHWWIG LHTSESDGRF 900
RWTDGSIINF ISWAPGKPRP VGKDKKCVYM TASREDWGDQ RCLTALPYIC KRSNVTKETQ 960
PPDLPTTALG GCPSDWIQFL NKCFQVQGQE PQSRVKWSEA QFSCEQQEAQ LVTITNPLEQ 1020
AFITASLPNV TFDLWIGLHA SQRDFQWVEQ EPLMYANWAP GEPSGPSPAP SGNKPTSCAV 1080
VLHSPSAHFT GRWDDRSCTE ETHGFICQKG TDPSLSPSPA ALPPAPGTEL SYLNGTFRLL 1140
QKPLRWHDAL LLCESRNASL AYVPDPYTQA FLTQAARGLR TPLWIGLAGE EGSRRYSWVS 1200
EEPLNYVGWQ DGEPQQPGGC TYVDVDGAWR TTSCDTKLQG AVCGVSSGPP PPRRISYHGS 1260
CPQGLADSAW IPFREHCYSF HMELLLGHKE ARQRCQRAGG AVLSILDEME NVFVWEHLQS 1320
YEGQSRGAWL GMNFNPKGGT LVWQDNTAVN YSNWGPPGLG PSMLSHNSCY WIQSNSGLWR 1380
PGACTNITMG VVCKLPRAEQ SSFSPSALPE NPAALVVVLM AVLLLLALLT AALILYRRRQ 1440
SIERGAFEGA RYSRSSSSPT EATEKNILVS DMEMNEQQE 1479 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW. 
Interpro
 IPR001304; C-type_lectin.
 IPR016186; C-type_lectin-like.
 IPR018378; C-type_lectin_CS.
 IPR016187; C-type_lectin_fold.
 IPR000562; FN_type2_col-bd.
 IPR013806; Kringle-like.
 IPR000772; Ricin_B_lectin. 
Pfam
 PF00040; fn2
 PF00059; Lectin_C 
SMART
 SM00034; CLECT
 SM00059; FN2
 SM00458; RICIN 
PROSITE
 PS00615; C_TYPE_LECTIN_1
 PS50041; C_TYPE_LECTIN_2
 PS00023; FN2_1
 PS51092; FN2_2
 PS50231; RICIN_B_LECTIN 
PRINTS