CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011609
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinetochore protein SLK19 
Protein Synonyms/Alias
 Synthetic lethal KAR3 protein 19 
Gene Name
 SLK19 
Gene Synonyms/Alias
 YOR195W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
120GGASTSDKHVLALNYacetylation[1]
233LENDINEKQQEEDFIubiquitination[2]
692DRITTLTKENEVLMEubiquitination[3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Has a role in spindle assembly and stability. Required to ensure a timely exit form mitosis. Essential to maintain pre- anaphase spindle polarity. Associates to the plus ends of the microtubules at the kinetochore and spindle midzone. A component of the FEAR (CDC14 Early Anaphase Release) network which promotes CDC14 release from the nucleolus during early anaphase. Required for proper chromosome segregation during meiosis I where it prevents premature sister chromatid seperation. 
Sequence Annotation
 MOD_RES 7 7 Phosphothreonine; by CDC28 (Probable).
 MOD_RES 188 188 Phosphoserine.
 MOD_RES 189 189 Phosphoserine.
 MOD_RES 201 201 Phosphoserine; alternate.
 MOD_RES 201 201 Phosphoserine; by CDC28; alternate
 MOD_RES 216 216 Phosphoserine.
 MOD_RES 273 273 Phosphothreonine.
 MOD_RES 283 283 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Meiosis; Microtubule; Mitosis; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 821 AA 
Protein Sequence
MNEVPTTPVR LILGQAQQRE QNSENCSQER NPRTFNSEPD SSFNSPGSSQ FVIHPHEPLE 60
KEKDEKQDLD RSIDYGRSSA LNNKNNANPL ENIDINKMFD DKKSDSGTND DKGGASTSDK 120
HVLALNYSPI RVEMNSSEKR SDKNVDVDEN DKEGSHINKK LKLQLESVPD LKQSSTKDII 180
NDKEEIMSSP MAIDMIETNI SPNKFIINDG VERNDSFNIN TDTLKLENDI NEKQQEEDFI 240
KSNSNNVVNI DNAYKEKEDE ENDITNSHIN RLTPLYETSA RESNSNEEGR NDYDDDNQLD 300
IRHDNFQIVA KRNEELTDQI YHLNQMLNSL ISKNESLSFQ YEKLNKNHQL LIDLTNEKLD 360
KLNTERESDI AKVEKFKKRI KELNTEIKVL NSNQKILQEK FDASITEVNH IKGEHENTVN 420
TLQQNEKILN DKNVELENMK AELKGNNDKL SEYETTLNDL NSRIVQLNDK IESTDIVLKS 480
KENELDNLKL SLKETLSISK DFNDSDLIGQ INELISTKNN LQQKMDDLNN LNDDNLKVVQ 540
DKLIKNEETL KLKEAEIDSL NSEMDELKKQ ITSKDDEFKM WQSKYETVED EAKIRNAEVT 600
ELNGDIEDLK ESKLHLEETI TELENKVHKL ENECELEKQK FEKTSLELES LQLKNSNIQA 660
EHIKELENLH ENLISLQNEL KISSDRITTL TKENEVLMEQ NNNNNNSVTL SNDQKDRDDE 720
KIKSLGKQVQ DWKEKYEAKE KDTNKRLKLL AEDLYIQYSS KHEQKVKLLK KGYENKYQNK 780
FDQLNLENKT LSEEIEQLNK QLSSEREEKQ ELLKLLENEK K 821 
Gene Ontology
 GO:0000778; C:condensed nuclear chromosome kinetochore; IDA:SGD.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0051233; C:spindle midzone; IDA:SGD.
 GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007126; P:meiosis; IMP:SGD.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0030472; P:mitotic spindle organization in nucleus; IMP:SGD. 
Interpro
 IPR024312; Kinetocho_Slk19. 
Pfam
 PF12709; Kinetocho_Slk19 
SMART
  
PROSITE
  
PRINTS