UniProt Accession

 PDP1_HUMAN; Q9P0J1; B3KX71; J3KPU0; Q5U5K1 

Genbank Protein ID

 AF155661; AK126862; DA769567; AC084346; CH471060; BC047619; BC098343 

Genbank Nucleotide ID

 AAF67480.1; BAG54383.1; EAW91704.1; AAH47619.1; AAH98343.1 

Protein Name

 [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial 

Protein Synonyms/Alias

 PDP 1; Protein phosphatase 2C; Pyruvate dehydrogenase phosphatase catalytic subunit 1; PDPC 1 

Gene Name

 PDP1 

Gene Synonyms/Alias

 PDP; PPM2C 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
77	RYASTPQKFYLTPPQ	ubiquitination	[1, 2]
90	PQVNSILKANEYSFK	ubiquitination	[1, 2]
97	KANEYSFKVPEFDGK	ubiquitination	[1, 2]
104	KVPEFDGKNVSSILG	ubiquitination	[1, 2]
194	LPILQWHKHPNDYFS	ubiquitination	[1, 2]
202	HPNDYFSKEASKLYF	acetylation	[3]
202	HPNDYFSKEASKLYF	ubiquitination	[1, 2]
206	YFSKEASKLYFNSLR	ubiquitination	[1, 2]
233	ESTDIDVKEALINAF	ubiquitination	[2]
241	EALINAFKRLDNDIS	ubiquitination	[1, 2]
326	ERELERLKLEHPKSE	ubiquitination	[1, 2]
331	RLKLEHPKSEAKSVV	ubiquitination	[2]
335	EHPKSEAKSVVKQDR	ubiquitination	[1]
339	SEAKSVVKQDRLLGL	ubiquitination	[1]
357	FRAFGDVKFKWSIDL	ubiquitination	[1, 2]
359	AFGDVKFKWSIDLQK	ubiquitination	[1, 2]
366	KWSIDLQKRVIESGP	ubiquitination	[1, 2]
383	LNDNEYTKFIPPNYH	ubiquitination	[1, 2]
411	HRLRPQDKFLVLATD	ubiquitination	[1, 2]
452	PIAVGGYKVTLGQMH	ubiquitination	[1, 2]
468	LLTERRTKMSSVFED	ubiquitination	[1, 2]
502	VDHERLSKMLSLPEE	ubiquitination	[1, 2, 4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex (By similarity). 

Sequence Annotation

 METAL 144 144 Magnesium 1 (By similarity).
 METAL 144 144 Magnesium 2 (By similarity).
 METAL 145 145 Magnesium 1; via carbonyl oxygen (By
 METAL 418 418 Magnesium 2 (By similarity).
 METAL 516 516 Magnesium 1 (By similarity).
 MOD_RES 202 202 N6-acetyllysine.  

Keyword

 Acetylation; Alternative splicing; Calcium; Complete proteome; Disease mutation; Hydrolase; Magnesium; Metal-binding; Mitochondrion; Protein phosphatase; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 537 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0004741; F:[pyruvate dehydrogenase (lipoamide)] phosphatase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:UniProtKB.
 GO:0035970; P:peptidyl-threonine dephosphorylation; IDA:UniProtKB.
 GO:0006090; P:pyruvate metabolic process; TAS:Reactome.
 GO:0010510; P:regulation of acetyl-CoA biosynthetic process from pyruvate; TAS:Reactome. 

Interpro

 IPR001932; PP2C-like.
 IPR000222; PP2C_Mn2_Asp60_BS.
 IPR015655; Protein_Pase_2C. 

Pfam

 PF00481; PP2C 

SMART

 SM00331; PP2C_SIG
 SM00332; PP2Cc 

PROSITE

 PS01032; PP2C 

PRINTS