UniProt Accession

 SEC17_YEAST; P32602; D6VPU9 

Genbank Protein ID

 M93104; Z23261; Z35811; BK006936 

Genbank Nucleotide ID

 AAA35029.1; CAA80796.1; CAA84870.1; DAA07069.1 

Protein Name

 Alpha-soluble NSF attachment protein 

Protein Synonyms/Alias

 SNAP-alpha; N-ethylmaleimide-sensitive factor attachment protein alpha; Vesicular-fusion protein SEC17; alpha-SNAP chaperone 

Gene Name

 SEC17 

Gene Synonyms/Alias

 YBL050W; YBL0505; YBL0517 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
186	DIYSKLIKSSMGNRL	ubiquitination	[1]
261	EQLSEHCKEFDNFMR	ubiquitination	[1, 2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 SNARE complex protein that binds to cis-SNARE complexes on membranes and is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus and for homotypic vacuole fusion. During the priming step of membrane fusion, is released from cis-SNARE complexes by SEC18 to establish a pool of unpaired SNAREs, which are required for interactions in trans during docking and fusion steps. Can displace HOPS from SNARE complexes, which may be a prerequisite for trans-SNARE complex disassembly and subsequent rounds of priming, docking and fusion. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; ER-Golgi transport; Membrane; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 292 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0019898; C:extrinsic to membrane; IDA:SGD.
 GO:0031201; C:SNARE complex; IPI:SGD.
 GO:0001671; F:ATPase activator activity; IDA:SGD.
 GO:0005483; F:soluble NSF attachment protein activity; IDA:SGD.
 GO:0006914; P:autophagy; IMP:SGD.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0035494; P:SNARE complex disassembly; IDA:SGD.
 GO:0042144; P:vacuole fusion, non-autophagic; IDA:SGD.
 GO:0048280; P:vesicle fusion with Golgi apparatus; IDA:SGD. 

Interpro

 IPR000744; NSF_attach.
 IPR011990; TPR-like_helical. 

Pfam

  

SMART

  

PROSITE

  

PRINTS

 PR00448; NSFATTACHMNT.