CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016816
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aminomethyltransferase, mitochondrial 
Protein Synonyms/Alias
 Glycine cleavage system T protein; GCVT 
Gene Name
 Amt 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
151SNAGCRDKDLALMQDacetylation[1, 2]
161ALMQDKVKEFQNRGLacetylation[2, 3]
357GCPSPSLKKNVAMGYacetylation[3]
368AMGYVPFKYSRPGTQacetylation[3, 4]
368AMGYVPFKYSRPGTQsuccinylation[4]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
 The glycine cleavage system catalyzes the degradation of glycine (By similarity). 
Sequence Annotation
 BINDING 232 232 Substrate (By similarity).
 BINDING 261 261 Substrate (By similarity).
 BINDING 399 399 Substrate (By similarity).  
Keyword
 Aminotransferase; Complete proteome; Mitochondrion; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 403 AA 
Protein Sequence
MHRIVSVVAP LGFRLQAQPL VQSRPLSSVQ DVLRRTPLYD FHLAHGGKMV AFAGWSLPVQ 60
YRDSHVDSHL HTRRHCSLFD VSHMLQTKIF GCDRVKLLES VVVGDIAELR PNQGTLSLFT 120
NEAGGILDDL IVSNTSEGHL YVVSNAGCRD KDLALMQDKV KEFQNRGLDV GLEVVENALL 180
ALQGPTATQV LQAGVTDDMK KLPFMTSAVM EVFGVSGCRV TRCGYTGEDG VEISVPATGA 240
VHLATTLLKN PEVKLAGLAA RDSLRLEAGL CLYGNDIDEH TTPVEGSLSW TLGKRRRIAM 300
DFPGAKIIVP QLKGEVQRRR VGLICEGAPV RAHSPILNTE GTVIGTVTSG CPSPSLKKNV 360
AMGYVPFKYS RPGTQLLVEV RRKQQMTVVS KMPFVPTNYY TLK 403 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004047; F:aminomethyltransferase activity; IEA:EC.
 GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
 GO:0006546; P:glycine catabolic process; IEA:InterPro. 
Interpro
 IPR013977; GCV_T_C.
 IPR006222; GCV_T_N.
 IPR006223; GcvT.
 IPR027266; TrmE/GcvT_dom1. 
Pfam
 PF01571; GCV_T
 PF08669; GCV_T_C 
SMART
  
PROSITE
  
PRINTS