UniProt Accession

 MED18_HUMAN; Q9BUE0; D3DPM1; Q9NXU9 

Genbank Protein ID

 AB107222; AK000052; AL353622; CH471059; CH471059; CH471059; BC002694 

Genbank Nucleotide ID

 BAD06869.1; BAA90910.1; CAI19132.1; EAX07699.1; EAX07700.1; EAX07702.1; AAH02694.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 18 

Protein Synonyms/Alias

 Mediator complex subunit 18; p28b 

Gene Name

 MED18 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
96	GQPEMGDKNRHALVR	ubiquitination	[1, 2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 

Sequence Annotation

  

Keyword

 Activator; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 208 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; IDA:MGI.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro. 

Interpro

 IPR019095; Mediator_Med18_met/fun. 

Pfam

 PF09637; Med18 

SMART

  

PROSITE

  

PRINTS