UniProt Accession

 PTNAB_ECOLI; P69797; P08186; Q47350 

Genbank Protein ID

 M36404; J02699; U00096; AP009048 

Genbank Nucleotide ID

 AAA24110.1; AAA24443.1; AAC74887.1; BAA15624.1 

Protein Name

 PTS system mannose-specific EIIAB component 

Protein Synonyms/Alias

 EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component 

Gene Name

 manX 

Gene Synonyms/Alias

 gptB; ptsL; b1817; JW1806 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
55	KYNAQLAKLDTTKGV	acetylation	[1, 2]
60	LAKLDTTKGVLFLVD	acetylation	[2]
132	GVKALKAKPVEKAAP	acetylation	[2]
136	LKAKPVEKAAPAPAA	acetylation	[2]
147	APAAAAPKAAPTPAK	acetylation	[2]
154	KAAPTPAKPMGPNDY	acetylation	[2]
184	QVATRWTKETNVSRI	acetylation	[2]
205	VAADTVRKTLLTQVA	acetylation	[2]
225	AHVVDVAKMIRVYNN	acetylation	[2, 3]
234	IRVYNNPKYAGERVM	acetylation	[1, 2, 3]
286	NAVSVDEKDIEAFKK	acetylation	[2]
292	EKDIEAFKKLNARGI	acetylation	[2]
293	KDIEAFKKLNARGIE	acetylation	[2]
305	GIELEVRKVSTDPKL	acetylation	[2]
311	RKVSTDPKLKMMDLI	acetylation	[2]
313	VSTDPKLKMMDLISK	acetylation	[2, 3]
320	KMMDLISKIDK****	acetylation	[2]

Reference

 [1] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannose transport. 

Sequence Annotation

 DOMAIN 2 124 PTS EIIA type-4.
 DOMAIN 157 320 PTS EIIB type-4.
 REGION 137 155 Hinge.
 ACT_SITE 10 10 Tele-phosphohistidine intermediate; for
 ACT_SITE 175 175 Pros-phosphohistidine intermediate; for
 MOD_RES 55 55 N6-acetyllysine.
 MOD_RES 234 234 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Cell inner membrane; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Membrane; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 323 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:EcoCyc.
 GO:0005887; C:integral to plasma membrane; IDA:EcoCyc.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0022870; F:protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity; IDA:EcoCyc.
 GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IDA:EcoCyc. 

Interpro

 IPR004701; PTS_EIIA_fruc.
 IPR013789; PTS_EIIA_fruc_subgr.
 IPR004720; PTS_IIB_sorbose-sp.
 IPR018455; PTS_IIB_sorbose-sp_subgr. 

Pfam

 PF03610; EIIA-man
 PF03830; PTSIIB_sorb 

SMART

  

PROSITE

 PS51096; PTS_EIIA_TYPE_4
 PS51101; PTS_EIIB_TYPE_4 

PRINTS