UniProt Accession

 DHB8_MOUSE; P50171; Q5M9K0; Q60958; Q60959; Q9Z1W2 

Genbank Protein ID

 U34072; U34072; AF100956; BC086927 

Genbank Nucleotide ID

 AAC53573.1; AAC53574.1; AAC69902.1; AAH86927.1 

Protein Name

 Estradiol 17-beta-dehydrogenase 8 

Protein Synonyms/Alias

 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; 3-oxoacyl-[acyl-carrier-protein] reductase; Protein Ke6; Ke-6; Testosterone 17-beta-dehydrogenase 8 

Gene Name

 Hsd17b8 

Gene Synonyms/Alias

 H2-Ke6; Hke6 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
66	EDGAPRGKHAAFQAD	acetylation	[1]

Reference

 [1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647] 

Functional Description

 NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (By similarity). The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria (By similarity). 

Sequence Annotation

 NP_BIND 13 21 NAD (By similarity).
 NP_BIND 40 41 NAD (By similarity).
 NP_BIND 72 74 NAD (By similarity).
 NP_BIND 167 171 NAD (By similarity).
 NP_BIND 200 202 NAD (By similarity).
 ACT_SITE 167 167 Proton acceptor (By similarity).
 BINDING 154 154 Substrate (By similarity).
 MOD_RES 66 66 N6-acetyllysine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Steroid biosynthesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 259 AA 

Protein Sequence

Gene Ontology

 GO:0005740; C:mitochondrial envelope; IDA:MGI.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:Compara.
 GO:0004303; F:estradiol 17-beta-dehydrogenase activity; ISS:UniProtKB.
 GO:0050327; F:testosterone 17-beta-dehydrogenase (NAD+) activity; IDA:MGI.
 GO:0047035; F:testosterone dehydrogenase (NAD+) activity; IEA:EC.
 GO:0008209; P:androgen metabolic process; IDA:MGI.
 GO:0006703; P:estrogen biosynthetic process; ISS:UniProtKB.
 GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

 PF00106; adh_short 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.