UniProt Accession

 BHMT1_MOUSE; O35490; Q3UEM1; Q3UL72; Q561N0 

Genbank Protein ID

 AH009623; AF033381; AK145668; AK149457; AK166754; AK166818; BC037004; BC093510; BC110307 

Genbank Nucleotide ID

 AAF85944.1; AAB87501.1; BAE26578.1; BAE28890.1; BAE38995.1; BAE39044.1; AAH37004.1; AAH93510.1; AAI10308.1 

Protein Name

 Betaine--homocysteine S-methyltransferase 1 

Protein Synonyms/Alias

  

Gene Name

 Bhmt 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
35	GFVFALEKRGYVKAG	ubiquitination	[1]
40	LEKRGYVKAGPWTPE	acetylation	[2]
40	LEKRGYVKAGPWTPE	succinylation	[2]
40	LEKRGYVKAGPWTPE	ubiquitination	[1]
82	TFYASEDKLENRGNY	ubiquitination	[1]
93	RGNYVAEKISGQKVN	acetylation	[2, 3, 4, 5]
93	RGNYVAEKISGQKVN	succinylation	[2]
93	RGNYVAEKISGQKVN	ubiquitination	[1]
98	AEKISGQKVNEAACD	acetylation	[2]
98	AEKISGQKVNEAACD	succinylation	[2]
98	AEKISGQKVNEAACD	ubiquitination	[1]
132	TPSYLSCKSEVEVKK	acetylation	[5, 6]
132	TPSYLSCKSEVEVKK	ubiquitination	[1]
138	CKSEVEVKKIFRQQL	acetylation	[3]
150	QQLEVFMKKNVDFLI	acetylation	[3, 5]
150	QQLEVFMKKNVDFLI	ubiquitination	[1]
207	ECAVRLVKAGASIVG	acetylation	[2, 6]
207	ECAVRLVKAGASIVG	succinylation	[2]
207	ECAVRLVKAGASIVG	ubiquitination	[1]
229	SVSLQTVKLMKEGLE	ubiquitination	[1]
232	LQTVKLMKEGLEAAR	acetylation	[2, 3, 6]
232	LQTVKLMKEGLEAAR	succinylation	[2]
232	LQTVKLMKEGLEAAR	ubiquitination	[1]
241	GLEAARLKAYLMSQP	acetylation	[2, 3, 6]
241	GLEAARLKAYLMSQP	succinylation	[2]
241	GLEAARLKAYLMSQP	ubiquitination	[1]
258	YHTPDCGKQGFIDLP	acetylation	[5]
283	ATRWDIQKYAREAYN	acetylation	[2, 3, 5, 6]
283	ATRWDIQKYAREAYN	succinylation	[2]
283	ATRWDIQKYAREAYN	ubiquitination	[1]
340	SGLDMHTKPWIRARA	acetylation	[2]
340	SGLDMHTKPWIRARA	succinylation	[2]
340	SGLDMHTKPWIRARA	ubiquitination	[1]
349	WIRARARKEYWQNLR	acetylation	[2]
349	WIRARARKEYWQNLR	succinylation	[2]
349	WIRARARKEYWQNLR	ubiquitination	[1]
377	PDAWGVTKGAAELMQ	acetylation	[2]
377	PDAWGVTKGAAELMQ	succinylation	[2]
377	PDAWGVTKGAAELMQ	ubiquitination	[1]
386	AAELMQQKEATTEQQ	acetylation	[2]
386	AAELMQQKEATTEQQ	succinylation	[2]
386	AAELMQQKEATTEQQ	ubiquitination	[1]
400	QLRELFEKQKFKSAQ	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758] 

Functional Description

 Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. 

Sequence Annotation

 DOMAIN 11 314 Hcy-binding.
 METAL 217 217 Zinc (By similarity).
 METAL 299 299 Zinc (By similarity).
 METAL 300 300 Zinc (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Metal-binding; Methyltransferase; Reference proteome; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 407 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0047150; F:betaine-homocysteine S-methyltransferase activity; IEA:EC.
 GO:0008898; F:homocysteine S-methyltransferase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006579; P:amino-acid betaine catabolic process; IEA:UniProtKB-UniPathway.
 GO:0009086; P:methionine biosynthetic process; IEA:InterPro. 

Interpro

 IPR017226; Betaine-hCys_S-MeTrfase_BHMT.
 IPR003726; S_MeTrfase. 

Pfam

 PF02574; S-methyl_trans 

SMART

  

PROSITE

 PS50970; HCY 

PRINTS