CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020330
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transmembrane protein 59 
Protein Synonyms/Alias
 Liver membrane-bound protein 
Gene Name
 TMEM59 
Gene Synonyms/Alias
 C1orf8; HSPC001; UNQ169/PRO195 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
63YPLHTYPKEEELYACubiquitination[1]
183KIVIFQSKPEIQYAPubiquitination[2]
206LRESSLSKMSYLQMRubiquitination[1, 2, 3]
287LEFMNEQKLNRYPASubiquitination[2]
302SLVVVRSKTEDHEEAubiquitination[1, 2]
315EAGPLPTKVNLAHSEubiquitination[1, 2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy. Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. Inhibits APP transport to the cell surface and further shedding. 
Sequence Annotation
 MOTIF 263 281 ATG16L1-binding motif.
 CARBOHYD 90 90 N-linked (GlcNAc...) (Probable).  
Keyword
 Autophagy; Cell membrane; Complete proteome; Endosome; Glycoprotein; Golgi apparatus; Lysosome; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 323 AA 
Protein Sequence
MAAPKGSLWV RTQLGLPPLL LLTMALAGGS GTASAEAFDS VLGDTASCHR ACQLTYPLHT 60
YPKEEELYAC QRGCRLFSIC QFVDDGIDLN RTKLECESAC TEAYSQSDEQ YACHLGCQNQ 120
LPFAELRQEQ LMSLMPKMHL LFPLTLVRSF WSDMMDSAQS FITSSWTFYL QADDGKIVIF 180
QSKPEIQYAP HLEQEPTNLR ESSLSKMSYL QMRNSQAHRN FLEDGESDGF LRCLSLNSGW 240
ILTTTLVLSV MVLLWICCAT VATAVEQYVP SEKLSIYGDL EFMNEQKLNR YPASSLVVVR 300
SKTEDHEEAG PLPTKVNLAH SEI 323 
Gene Ontology
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005770; C:late endosome; IDA:UniProtKB.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0004175; F:endopeptidase activity; IEA:Compara.
 GO:0006914; P:autophagy; IEA:UniProtKB-KW.
 GO:0010508; P:positive regulation of autophagy; IMP:UniProtKB. 
Interpro
 IPR022065; Uncharacterised_TMEM59. 
Pfam
 PF12280; BSMAP 
SMART
  
PROSITE
  
PRINTS