CPLM-002364

Genbank Nucleotide ID

Eukaryotic translation initiation factor 4E

Protein Synonyms/Alias

eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Homo sapiens (Human)

Position	Peptide	Type	References
36	ANPEHYIKHPLQNRW	ubiquitination	[1]
54	FFKNDKSKTWQANLR	ubiquitination	[2]
119	RWLITLNKQQRRSDL	ubiquitination	[2, 3, 4]
193	NVRAKGDKIAIWTTE	ubiquitination	[2]
237	SHADTATKSGSTTKN	ubiquitination	[2]

[1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

REGION 37 40 EIF4EBP1/2/3 binding.
REGION 56 57 7-methylguanosine-containing mRNA cap
REGION 73 77 EIF4EBP1/2/3 binding.
REGION 102 103 7-methylguanosine-containing mRNA cap
REGION 132 139 EIF4EBP1/2/3 binding.
REGION 157 162 7-methylguanosine-containing mRNA cap
REGION 205 207 7-methylguanosine-containing mRNA cap
MOD_RES 2 2 N-acetylalanine.
MOD_RES 209 209 Phosphoserine; by PKC and MKNK2.

3D-structure; Acetylation; Alternative splicing; Chromosomal rearrangement; Complete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000932; C:cytoplasmic mRNA processing body; IDA:MGI.
GO:0010494; C:cytoplasmic stress granule; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:ProtInc.
GO:0005845; C:mRNA cap binding complex; IDA:UniProtKB.
GO:0016442; C:RNA-induced silencing complex; IDA:MGI.
GO:0000339; F:RNA cap binding; TAS:ProtInc.
GO:0003743; F:translation initiation factor activity; ISS:UniProtKB.
GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:UniProtKB.
GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
GO:0030324; P:lung development; IEA:Compara.
GO:0006406; P:mRNA export from nucleus; TAS:Reactome.
GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
GO:0045931; P:positive regulation of mitotic cell cycle; IMP:UniProtKB.
GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR023398; TIF_eIF4e-like_dom.
IPR001040; TIF_eIF_4E.
IPR019770; TIF_eIF_4E_CS.