CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010837
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Runt-related transcription factor 1 
Protein Synonyms/Alias
 Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit 
Gene Name
 RUNX1 
Gene Synonyms/Alias
 AML1; CBFA2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51STALSPGKMSEALPLubiquitination[1]
70AGAALAGKLRSGDRSubiquitination[1, 2, 3, 4]
110PTHWRCNKTLPIAFKubiquitination[1]
152RNATAAMKNQVARFNubiquitination[1, 2, 3]
194ATYHRAIKITVDGPRubiquitination[1]
209EPRRHRQKLDDQTKPubiquitination[1, 2]
215QKLDDQTKPGSLSFSubiquitination[1, 2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. 
Sequence Annotation
 DOMAIN 50 178 Runt.
 REGION 80 84 Interaction with DNA.
 REGION 135 143 Interaction with DNA.
 REGION 168 177 Interaction with DNA.
 REGION 291 371 Interaction with KAT6A.
 REGION 307 400 Interaction with KAT6B (By similarity).
 BINDING 112 112 Chloride 1.
 BINDING 116 116 Chloride 1; via amide nitrogen.
 BINDING 139 139 Chloride 2.
 BINDING 170 170 Chloride 2; via amide nitrogen.
 MOD_RES 14 14 Phosphothreonine.
 MOD_RES 21 21 Phosphoserine.
 MOD_RES 24 24 N6-acetyllysine.
 MOD_RES 43 43 N6-acetyllysine.
 MOD_RES 249 249 Phosphoserine; by HIPK2.
 MOD_RES 266 266 Phosphoserine.
 MOD_RES 268 268 Phosphoserine.
 MOD_RES 273 273 Phosphothreonine; by HIPK2.
 MOD_RES 276 276 Phosphoserine; by HIPK2.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Chloride; Chromosomal rearrangement; Complete proteome; Disease mutation; DNA-binding; Methylation; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 453 AA 
Protein Sequence
MASDSIFESF PSYPQCFMRE CILGMNPSRD VHDASTSRRF TPPSTALSPG KMSEALPLGA 60
PDAGAALAGK LRSGDRSMVE VLADHPGELV RTDSPNFLCS VLPTHWRCNK TLPIAFKVVA 120
LGDVPDGTLV TVMAGNDENY SAELRNATAA MKNQVARFND LRFVGRSGRG KSFTLTITVF 180
TNPPQVATYH RAIKITVDGP REPRRHRQKL DDQTKPGSLS FSERLSELEQ LRRTAMRVSP 240
HHPAPTPNPR ASLNHSTAFN PQPQSQMQDT RQIQPSPPWS YDQSYQYLGS IASPSVHPAT 300
PISPGRASGM TTLSAELSSR LSTAPDLTAF SDPRQFPALP SISDPRMHYP GAFTYSPTPV 360
TSGIGIGMSA MGSATRYHTY LPPPYPGSSQ AQGGPFQASS PSYHLYYGAS AGSYQFSMVG 420
GERSPPRILP PCTNASTGSA LLNPSLPNQS DVVEAEGSHS NSPTNMAPSA RLEEAVWRPY 480 
Gene Ontology
 GO:0005604; C:basement membrane; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0000975; F:regulatory region DNA binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0008134; F:transcription factor binding; IDA:UniProtKB.
 GO:0048266; P:behavioral response to pain; IEA:Compara.
 GO:0007417; P:central nervous system development; IEA:Compara.
 GO:0060216; P:definitive hemopoiesis; IEA:Compara.
 GO:0035162; P:embryonic hemopoiesis; IEA:Compara.
 GO:0031069; P:hair follicle morphogenesis; IEA:Compara.
 GO:0071425; P:hematopoietic stem cell proliferation; TAS:UniProtKB.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0030099; P:myeloid cell differentiation; IDA:UniProtKB.
 GO:0002318; P:myeloid progenitor cell differentiation; IEA:Compara.
 GO:0030853; P:negative regulation of granulocyte differentiation; IMP:UniProtKB.
 GO:0048935; P:peripheral nervous system neuron development; TAS:BHF-UCL.
 GO:0045766; P:positive regulation of angiogenesis; ISS:UniProtKB.
 GO:0030854; P:positive regulation of granulocyte differentiation; IMP:UniProtKB.
 GO:2000872; P:positive regulation of progesterone secretion; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0071336; P:regulation of hair follicle cell proliferation; IEA:Compara.
 GO:0009966; P:regulation of signal transduction; IEA:Compara.
 GO:0001501; P:skeletal system development; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000040; AML1_Runt.
 IPR008967; p53-like_TF_DNA-bd.
 IPR012346; p53/RUNT-type_TF_DNA-bd.
 IPR013524; Runt_dom.
 IPR027384; Runx_central_dom.
 IPR013711; RunxI_C_dom.
 IPR016554; TF_Runt-rel_RUNX. 
Pfam
 PF00853; Runt
 PF08504; RunxI 
SMART
  
PROSITE
 PS51062; RUNT 
PRINTS
 PR00967; ONCOGENEAML1.