UniProt Accession

 NFNB_ECOLI; P38489; P19575 

Genbank Protein ID

 D25414; U07860; U82598; U00096; AP009048 

Genbank Nucleotide ID

 BAA05004.1; AAC43263.1; AAB40776.1; AAC73679.1; BAA35218.1 

Protein Name

 Oxygen-insensitive NAD(P)H nitroreductase 

Protein Synonyms/Alias

 Dihydropteridine reductase; FMN-dependent nitroreductase 

Gene Name

 nfnB 

Gene Synonyms/Alias

 dprA; nfsB; nfsI; ntr; b0578; JW0567 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
14	ALKRHSTKAFDASKK	acetylation	[1, 2]
20	TKAFDASKKLTPEQA	acetylation	[2]
21	KAFDASKKLTPEQAE	pupylation	[3]
62	EGKARVAKSAAGNYV	pupylation	[3]
181	AEFGLKEKGYTSLVV	acetylation	[2]
205	DFNATLPKSRLPQNI	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 

Functional Description

 Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5- (aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. 

Sequence Annotation

 NP_BIND 10 14 FMN.
 NP_BIND 153 158 NAD or NADP (By similarity).
 NP_BIND 165 166 FMN.
 NP_BIND 205 207 FMN.
 BINDING 41 41 NAD or NADP; via amide nitrogen.
 BINDING 71 71 FMN.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Flavoprotein; FMN; NAD; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 217 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0004155; F:6,7-dihydropteridine reductase activity; IDA:EcoCyc.
 GO:0071949; F:FAD binding; IDA:EcoCyc.
 GO:0010181; F:FMN binding; IDA:EcoCyc.
 GO:0018545; F:NAD(P)H nitroreductase activity; IMP:EcoCyc. 

Interpro

 IPR000415; Nitroreductase-like. 

Pfam

 PF00881; Nitroreductase 

SMART

  

PROSITE

  

PRINTS