CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021369
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Golgi phosphoprotein 3 
Protein Synonyms/Alias
 Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS 
Gene Name
 GOLPH3 
Gene Synonyms/Alias
 GPP34 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56DDDKGDSKETRLTLMubiquitination[1]
123LTRKVICKSDAPTGDubiquitination[2]
138VLLDEALKHVKETQPubiquitination[2]
179NVRERLAKNLVEKGVubiquitination[2]
191KGVLTTEKQNFLLFDubiquitination[3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. 
Sequence Annotation
 REGION 190 201 Beta-hairpin required for oligomerization
 BINDING 81 81 PtdIns4P (Probable).
 BINDING 90 90 PtdIns4P (Probable).
 BINDING 171 171 PtdIns4P (Probable).
 BINDING 174 174 PtdIns4P (Probable).
 MOD_RES 36 36 Phosphoserine.
 DISULFID 84 108  
Keyword
 3D-structure; Cell membrane; Complete proteome; Cytoplasm; Disulfide bond; Endosome; Golgi apparatus; Lipid-binding; Membrane; Mitochondrion; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 298 AA 
Protein Sequence
MTSLTQRSSG LVQRRTEASR NAADKERAAG GGAGSSEDDA QSRRDEQDDD DKGDSKETRL 60
TLMEEVLLLG LKDREGYTSF WNDCISSGLR GCMLIELALR GRLQLEACGM RRKSLLTRKV 120
ICKSDAPTGD VLLDEALKHV KETQPPETVQ NWIELLSGET WNPLKLHYQL RNVRERLAKN 180
LVEKGVLTTE KQNFLLFDMT THPLTNNNIK QRLIKKVQEA VLDKWVNDPH RMDRRLLALI 240
YLAHASDVLE NAFAPLLDEQ YDLATKRVRQ LLDLDPEVEC LKANTNEVLW AVVAAFTK 298 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; IMP:UniProtKB.
 GO:0032008; P:positive regulation of TOR signaling cascade; IMP:UniProtKB.
 GO:0010821; P:regulation of mitochondrion organization; IDA:UniProtKB. 
Interpro
 IPR008628; GPP34. 
Pfam
 PF05719; GPP34 
SMART
  
PROSITE
  
PRINTS