CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012087
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrin-linked protein kinase 
Protein Synonyms/Alias
 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK 
Gene Name
 ILK 
Gene Synonyms/Alias
 ILK1; ILK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
85GHRDIVQKLLQYKADubiquitination[1, 2, 3, 4, 5, 6, 7]
139YGEMPVDKAKAPLREubiquitination[2, 5, 6]
141EMPVDKAKAPLRELLubiquitination[5]
154LLRERAEKMGQNLNRubiquitination[5, 8]
165NLNRIPYKDTFWKGTubiquitination[1, 3, 5, 7, 8]
170PYKDTFWKGTTRTRPubiquitination[2, 8]
184PRNGTLNKHSGIDFKubiquitination[5]
191KHSGIDFKQLNFLTKubiquitination[1, 2, 3, 5, 6, 7, 8]
198KQLNFLTKLNENHSGubiquitination[2, 5]
209NHSGELWKGRWQGNDubiquitination[2, 5, 6]
220QGNDIVVKVLKVRDWubiquitination[1, 2, 7, 8]
341RISMADVKFSFQCPGubiquitination[3, 5]
363VAPEALQKKPEDTNRubiquitination[2]
426PHVCKLMKICMNEDPubiquitination[5]
438EDPAKRPKFDMIVPIubiquitination[1, 2, 5, 6, 7, 8]
448MIVPILEKMQDK***ubiquitination[2, 5, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. 
Sequence Annotation
 REPEAT 2 30 ANK 1.
 REPEAT 31 63 ANK 2.
 REPEAT 64 96 ANK 3.
 REPEAT 97 129 ANK 4.
 REPEAT 130 174 ANK 5.
 DOMAIN 193 446 Protein kinase.
 NP_BIND 199 207 ATP (By similarity).
 REGION 33 139 Interaction with LIMS1.
 REGION 180 212 PH-like; mediates interaction with
 BINDING 220 220 ATP (By similarity).
 MOD_RES 186 186 Phosphoserine.  
Keyword
 3D-structure; ANK repeat; ATP-binding; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 452 AA 
Protein Sequence
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV EMLIMRGARI 60
NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG NVPLHYACFW GQDQVAEDLV 120
ANGALVSICN KYGEMPVDKA KAPLRELLRE RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG 180
TLNKHSGIDF KQLNFLTKLN ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP 240
RLRIFSHPNV LPVLGACQSP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD 300
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM YAPAWVAPEA 360
LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI GMKVALEGLR PTIPPGISPH 420
VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK 452 
Gene Ontology
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0043034; C:costamere; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0043198; C:dendritic shaft; IEA:Compara.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0030017; C:sarcomere; IEA:UniProtKB-SubCell.
 GO:0043195; C:terminal bouton; IEA:Compara.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; IEA:Compara.
 GO:0001658; P:branching involved in ureteric bud morphogenesis; IEA:Compara.
 GO:0007569; P:cell aging; IEA:Compara.
 GO:0007050; P:cell cycle arrest; IEA:Compara.
 GO:0034329; P:cell junction assembly; TAS:Reactome.
 GO:0008283; P:cell proliferation; NAS:UniProtKB.
 GO:0007160; P:cell-matrix adhesion; NAS:UniProtKB.
 GO:0045197; P:establishment or maintenance of epithelial cell apical/basal polarity; IEA:Compara.
 GO:0043206; P:extracellular fibril organization; IEA:Compara.
 GO:0007229; P:integrin-mediated signaling pathway; NAS:UniProtKB.
 GO:0032288; P:myelin assembly; IEA:Compara.
 GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0006469; P:negative regulation of protein kinase activity; IEA:Compara.
 GO:0014912; P:negative regulation of smooth muscle cell migration; IEA:Compara.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0048812; P:neuron projection morphogenesis; IEA:Compara.
 GO:0018105; P:peptidyl-serine phosphorylation; IEA:Compara.
 GO:0045773; P:positive regulation of axon extension; IEA:Compara.
 GO:0030513; P:positive regulation of BMP signaling pathway; IEA:Compara.
 GO:0090263; P:positive regulation of canonical Wnt receptor signaling pathway; IMP:UniProtKB.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0001954; P:positive regulation of cell-matrix adhesion; IEA:Compara.
 GO:0050775; P:positive regulation of dendrite morphogenesis; IEA:Compara.
 GO:0043406; P:positive regulation of MAP kinase activity; IEA:Compara.
 GO:0045663; P:positive regulation of myoblast differentiation; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Compara.
 GO:0042327; P:positive regulation of phosphorylation; IMP:UniProtKB.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0032956; P:regulation of actin cytoskeleton organization; IEA:Compara.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IMP:UniProtKB. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR016253; Integrin-linked_kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom. 
Pfam
 PF12796; Ank_2
 PF07714; Pkinase_Tyr 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS