CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008643
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 2A 
Protein Synonyms/Alias
 eIF-2A 
Gene Name
 YGR054W 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
55LSTKENVKVFTGPCLubiquitination[1]
109VKVWYLNKPFKKDCVacetylation[2]
383ATNTSVCKWSPGGEFubiquitination[1]
615ERQAVGDKLEDTQVLubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. Specifically associates with both 40S subunits and 80 S ribosomes. 
Sequence Annotation
 REPEAT 69 115 WD 1.
 REPEAT 186 224 WD 2.
 REPEAT 289 331 WD 3.
 REPEAT 374 419 WD 4.
 MOD_RES 564 564 Phosphoserine.
 MOD_RES 567 567 Phosphoserine.
 MOD_RES 572 572 Phosphoserine.  
Keyword
 Complete proteome; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; Translation regulation; Ubl conjugation; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 642 AA 
Protein Sequence
MSSQFFLKTS QDIELFQSYP TFEQSNTNSK DFPVISSVLS PCGRFLALST KENVKVFTGP 60
CLDNVLLTMK LSDVYDLHFS PAGNYLSTWE RASIQDPNHK NVKVWYLNKP FKKDCVSEDI 120
VPAYEYQAKS QSGWFLQFSK LDNYGLRLFK HDLKIVKLSS ANADNFDFQS PFAVLSDDET 180
SQHFTTYLIS PAEHPTICTF TPEKGGKPAQ LIIWALSEGK ITKKIASKTF FKADSCQLKW 240
NPLGNAILCL AITDFDSSNK SYYGENTLYL LSFQGVNGTL GGNSVRVSLT TGPVHDFTWS 300
PTSRQFGVIA GYMPATISFF DLRGNVVHSL PQQAKNTMLF SPSGHYILIA GFGNLQGSVE 360
ILDRLDKFKC VSKFDATNTS VCKWSPGGEF IMTATTSPRL RVDNGVKIWH VSGSLVFVKE 420
FKELLKVDWR SPCNYKTLEN KDEAFFENHI INNWEPLPDS TTSSLDPKIS NKSELQIHSS 480
VQEYISQHPS REASSNGNGS KAKAGGAYKP PHARRTGGGR IVPGVPPGAA KKTIPGLVPG 540
MSANKDANTK NRRRRANKKS SETSPDSTPA PSAPASTNAP TNNKETSPEE KKIRSLLKKL 600
RAIETLKERQ AVGDKLEDTQ VLKIQTEEKV LKDLEKLGWK DE 642 
Gene Ontology
 GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; IDA:SGD.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 
Interpro
 IPR011387; TIF2A.
 IPR013979; TIF_beta_prop-like.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08662; eIF2A 
SMART
  
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS