CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020442
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Activating molecule in BECN1-regulated autophagy protein 1 
Protein Synonyms/Alias
  
Gene Name
 AMBRA1 
Gene Synonyms/Alias
 KIAA1736 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MKVVPEKNAVRILWubiquitination[1]
36LQELVEDKTRWMKWEubiquitination[1]
41EDKTRWMKWEGKRVEubiquitination[1]
175REPFAVVKTASEMERubiquitination[1, 2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity). 
Sequence Annotation
 REPEAT 51 90 WD 1.
 REPEAT 93 133 WD 2.
 REPEAT 135 175 WD 3.
 MOD_RES 639 639 Phosphoserine.  
Keyword
 Alternative splicing; Autophagy; Complete proteome; Cytoplasmic vesicle; Developmental protein; Differentiation; Neurogenesis; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1298 AA 
Protein Sequence
MKVVPEKNAV RILWGRERGA RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP DSPRSTFLLA 60
FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC VTFHPTISGL IASGCLDGEV 120
RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ LLLIATANEI HFWDWSRREP FAVVKTASEM 180
ERVRLVRFDP LGHYLLTAIV NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL 240
HNFLHMLSSR SSGIQTEPFH PPEQASSTQQ DQGLLNRPSA FSTVQSSTAG NTLRNLSLGP 300
TRRSLGGPLS SHPSRYHREI APGLTGSEWT RTVLSLNSRS EAESMPPPRT SASSVSLLSV 360
LRQQEGGSQA SVYTSATEGR GFPASGLATE SDGGNGSSQN NSGSIRHELQ CDLRRFFLEY 420
DRLQELDQSL SGEAPQTQQA QEMLNNNIES ERPGPSHQPT PHSSENNSNL SRGHLNRCRA 480
CHNLLTFNND TLRWERTTPN YSSGEASSSW QVPSSFESVP SSGSQLPPLE RTEGQTPSSS 540
RLELSSSASP QEERTVGVAF NQETGHWERI YTQSSRSGTV SQEALHQDMP EESSEEDSLR 600
RRSLALSPRL EYSGAILAHC KLRLPGSCHS PASASQVAGT TGAHHHARLI FAFLVEMEFH 660
HVSQAGLELL TSGDLPTSAS QSAGITGVSH RAWPRLLESS LISLSRYDGA GSREHPIYPD 720
PARLSPAAYY AQRMIQYLSR RDSIRQRSMR YQQNRLRSST SSSSSDNQGP SVEGTDLEFE 780
DFEDNGDRSR HRAPRNARMS APSLGRFVPR RFLLPEYLPY AGIFHERGQP GLATHSSVNR 840
VLAGAVIGDG QSAVASNIAN TTYRLQWWDF TKFDLPEISN ASVNVLVQNC KIYNDASCDI 900
SADGQLLAAF IPSSQRGFPD EGILAVYSLA PHNLGEMLYT KRFGPNAISV SLSPMGRYVM 960
VGLASRRILL HPSTEHMVAQ VFRLQQAHGG ETSMRRVFNV LYPMPADQRR HVSINSARWL 1020
PEPGLGLAYG TNKGDLVICR PEALNSGVEY YWDQLNETVF TVHSNSRSSE RPGTSRATWR 1080
TDRDMGLMNA IGLQPRNPAT SVTSQGTQTL ALQLQNAETQ TEREVPEPGT AASGPGEGEG 1140
SEYGASGEDA LSRIQRLMAE GGMTAVVQRE QSTTMASMGG FGNNIIVSHR IHRSSQTGTE 1200
PGAAHTSSPQ PSTSRGLLPE AGQLAERGLS PRTASWDQPG TPGREPTQPT LPSSSPVPIP 1260
VSLPSAEGPT LHCELTNNNH LLDGGSSRGD AAGPRGEPRN R 1301 
Gene Ontology
 GO:0005776; C:autophagic vacuole; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
 GO:0006914; P:autophagy; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0021915; P:neural tube development; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS