UniProt Accession

 HIS5_ECOLI; P60595; P10375 

Genbank Protein ID

 X13462; U00096; AP009048 

Genbank Nucleotide ID

 CAA31815.1; AAC75084.1; BAA15854.1 

Protein Name

 Imidazole glycerol phosphate synthase subunit HisH 

Protein Synonyms/Alias

 IGP synthase glutamine amidotransferase subunit; IGP synthase subunit HisH; ImGP synthase subunit HisH; IGPS subunit HisH 

Gene Name

 hisH 

Gene Synonyms/Alias

 b2023; JW2005 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
28	ARHGYEPKVSRDPDV	acetylation	[1]
188	RSGAAGAKLLKNFLE	acetylation	[1]
191	AAGAKLLKNFLEM**	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR. 

Sequence Annotation

 DOMAIN 2 196 Glutamine amidotransferase type-1.
 ACT_SITE 77 77 Nucleophile (By similarity).
 ACT_SITE 178 178 By similarity.
 ACT_SITE 180 180 By similarity.  

Keyword

 Amino-acid biosynthesis; Complete proteome; Cytoplasm; Direct protein sequencing; Glutamine amidotransferase; Histidine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 196 AA 

Protein Sequence

Gene Ontology

 GO:0009382; C:imidazoleglycerol-phosphate synthase complex; IDA:EcoCyc.
 GO:0000107; F:imidazoleglycerol-phosphate synthase activity; IDA:EcoCyc.
 GO:0006541; P:glutamine metabolic process; IEA:HAMAP.
 GO:0000105; P:histidine biosynthetic process; IDA:EcoCyc. 

Interpro

 IPR017926; GATASE.
 IPR010139; Imidazole-glycPsynth_HisH. 

Pfam

 PF00117; GATase 

SMART

  

PROSITE

 PS51273; GATASE_TYPE_1 

PRINTS