CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012162
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 KRR1 small subunit processome component homolog 
Protein Synonyms/Alias
 HIV-1 Rev-binding protein 2; KRR-R motif-containing protein 1; Rev-interacting protein 1; Rip-1 
Gene Name
 KRR1 
Gene Synonyms/Alias
 HRB2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24EFRNQKPKPENQDESubiquitination[1]
41LTVPDGWKEPAFSKEubiquitination[1]
106MTVCTTKKTFDPYIIubiquitination[1, 2, 3, 4, 5]
121IRARDLIKLLARSVSubiquitination[2, 4, 5]
146DVACDIIKIGSLVRNubiquitination[4]
168RQRLIGPKGSTLKALubiquitination[2, 4, 5, 6]
220IHPIYNIKSLMIKREubiquitination[2, 4, 5]
230MIKRELAKDSELRSQubiquitination[4]
280QPESQIDKELASGEYubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity). 
Sequence Annotation
 DOMAIN 154 206 KH.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 3 3 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ribonucleoprotein; Ribosome biogenesis; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 381 AA 
Protein Sequence
MASPSLERPE KGAGKSEFRN QKPKPENQDE SELLTVPDGW KEPAFSKEDN PRGLLEESSF 60
ATLFPKYREA YLKECWPLVQ KALNEHHVNA TLDLIEGSMT VCTTKKTFDP YIIIRARDLI 120
KLLARSVSFE QAVRILQDDV ACDIIKIGSL VRNKERFVKR RQRLIGPKGS TLKALELLTN 180
CYIMVQGNTV SAIGPFSGLK EVRKVVLDTM KNIHPIYNIK SLMIKRELAK DSELRSQSWE 240
RFLPQFKHKN VNKRKEPKKK TVKKEYTPFP PPQPESQIDK ELASGEYFLK ANQKKRQKME 300
AIKAKQAEAI SKRQEERNKA FIPPKEKPIV KPKEASTETK IDVASIKEKV KKAKNKKLGA 360
LTAEEIALKM EADEKKKKKK K 381 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR004087; KH_dom.
 IPR004088; KH_dom_type_1.
 IPR024166; rRNA_assembly_KRR1. 
Pfam
 PF00013; KH_1 
SMART
 SM00322; KH 
PROSITE
 PS50084; KH_TYPE_1 
PRINTS