CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005203
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription elongation factor SPT6 
Protein Synonyms/Alias
 Chromatin elongation factor SPT6 
Gene Name
 SPT6 
Gene Synonyms/Alias
 CRE2; SSN20; YGR116W; G6169 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
118KASSSSGKFKRLKRVacetylation[1]
229LLREQSIKQPTQITGacetylation[1]
491MFINHTGKTGKKHLKacetylation[1]
1007SWNEKRQKYEDLEHDacetylation[1]
1134ESEKTFFKGSIIPVRacetylation[1]
1355NEMTSSEKFKSGTKKacetylation[1]
1355NEMTSSEKFKSGTKKubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability. 
Sequence Annotation
 DOMAIN 1257 1354 SH2.
 MOTIF 8 12 Nuclear localization signal (Potential).
 MOTIF 77 85 Nuclear localization signal (Potential).
 MOTIF 120 125 Nuclear localization signal (Potential).
 MOD_RES 94 94 Phosphoserine.
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 136 136 Phosphoserine.
 MOD_RES 148 148 Phosphoserine.
 MOD_RES 155 155 Phosphoserine.
 MOD_RES 206 206 Phosphoserine.
 MOD_RES 295 295 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; SH2 domain; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1451 AA 
Protein Sequence
MEETGDSKLV PRDEEEIVND NDETKAPSEE EEGEDVFDSS EEDEDIDEDE DEARKVQEGF 60
IVNDDDENED PGTSISKKRR KHKRREREED DRLSEDDLDL LMENAGVERT KASSSSGKFK 120
RLKRVGDEGN AAESESDNVA ASRQDSTSKL EDFFSEDEEE EESGLRNGRN NEYGRDEEDH 180
ENRNRTADKG GILDELDDFI EDDEFSDEDD ETRQRRIQEK KLLREQSIKQ PTQITGLSSD 240
KIDEMYDIFG DGHDYDWALE IENEELENGN DNNEAEEEEI DEETGAIKST KKKISLQDIY 300
DLEDLKKNLM TEGDMKIRKT DIPERYQELR AGITDYGNMS SEDQELERNW IAEKISVDKN 360
FDANYDLTEF KEAIGNAIKF ITKENLEVPF IYAYRRNYIS SREKDGFLLT EDDLWDIVSL 420
DIEFHSLVNK KDYVQRFYAE LHIDDPIVTE YFKNQNTASI AELNSLQDIY DYLEFKYANE 480
INEMFINHTG KTGKKHLKNS SYEKFKASPL YQAVSDIGIS AEDVGENISS QHQIHPPVDH 540
PSSKPVEVIE SILNANSGDL QVFTSNTKLA IDTVQKYYSL ELSKNTKIRE KVRSDFSKYY 600
LADVVLTAKG KKEIQKGSLY EDIKYAINRT PMHFRRDPDV FLKMVEAESL NLLSVKLHMS 660
SQAQYIEHLF QIALETTNTS DIAIEWNNFR KLAFNQAMDK IFQDISQEVK DNLTKNCQKL 720
VAKTVRHKFM TKLDQAPFIP NVRDPKIPKI LSLTCGQGRF GADAIIAVYV NRKGDFIRDY 780
KIVDNPFDKT NPEKFEDTLD NIIQSCQPNA IGINGPNPKT QKFYKRLQEV LHKKQIVDSR 840
GHTIPIIYVE DEVAIRYQNS ERAAQEFPNK PPLVKYCIAL ARYMHSPLLE YANLTSEEVR 900
SLSIHPHQNL LSSEQLSWAL ETAFVDIVNL VSVEVNKATD NNYYASALKY ISGFGKRKAI 960
DFLQSLQRLN EPLLARQQLI THNILHKTIF MNSAGFLYIS WNEKRQKYED LEHDQLDSTR 1020
IHPEDYHLAT KVAADALEYD PDTIAEKEEQ GTMSEFIELL REDPDRRAKL ESLNLESYAE 1080
ELEKNTGLRK LNNLNTIVLE LLDGFEELRN DFHPLQGDEI FQSLTGESEK TFFKGSIIPV 1140
RVERFWHNDI ICTTNSEVEC VVNAQRHAGA QLRRPANEIY EIGKTYPAKV IYIDYANITA 1200
EVSLLDHDVK QQYVPISYSK DPSIWDLKQE LEDAEEERKL MMAEARAKRT HRVINHPYYF 1260
PFNGRQAEDY LRSKERGEFV IRQSSRGDDH LVITWKLDKD LFQHIDIQEL EKENPLALGK 1320
VLIVDNQKYN DLDQIIVEYL QNKVRLLNEM TSSEKFKSGT KKDVVKFIED YSRVNPNKSV 1380
YYFSLNHDNP GWFYLMFKIN ANSKLYTWNV KLTNTGYFLV NYNYPSVIQL CNGFKTLLKS 1440
NSSKNRMNNY R 1451 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0035327; C:transcriptionally active chromatin; IDA:SGD.
 GO:0000991; F:core RNA polymerase II binding transcription factor activity; IMP:SGD.
 GO:0001073; F:DNA binding transcription antitermination factor activity; IMP:SGD.
 GO:0042393; F:histone binding; IDA:SGD.
 GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
 GO:0031491; F:nucleosome binding; IMP:SGD.
 GO:0070827; P:chromatin maintenance; IMP:SGD.
 GO:0006338; P:chromatin remodeling; IMP:SGD.
 GO:0000433; P:negative regulation of transcription from RNA polymerase II promoter by glucose; IMP:SGD.
 GO:0006334; P:nucleosome assembly; IDA:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD.
 GO:0071931; P:positive regulation of transcription involved in G1/S phase of mitotic cell cycle; IMP:SGD.
 GO:0000414; P:regulation of histone H3-K36 methylation; IMP:SGD.
 GO:0031440; P:regulation of mRNA 3'-end processing; IMP:SGD.
 GO:0060303; P:regulation of nucleosome density; IMP:SGD.
 GO:0034401; P:regulation of transcription by chromatin organization; IMP:SGD.
 GO:0043618; P:regulation of transcription from RNA polymerase II promoter in response to stress; IMP:SGD.
 GO:0001178; P:regulation of transcriptional start site selection at RNA polymerase II promoter; IMP:SGD. 
Interpro
 IPR000980; SH2.
 IPR023323; Tex-like_dom.
 IPR023097; Tex_RuvX-like_dom.
 IPR017072; TF_Spt6.
 IPR006641; YqgF/RNaseH-like_dom. 
Pfam
 PF00017; SH2 
SMART
 SM00252; SH2
 SM00732; YqgFc 
PROSITE
 PS50001; SH2 
PRINTS