UniProt Accession

 GRPE_ECOLI; P09372 

Genbank Protein ID

 X07863; U00096; AP009048 

Genbank Nucleotide ID

 CAA30711.1; AAC75663.1; BAA16498.1 

Protein Name

 Protein GrpE 

Protein Synonyms/Alias

 HSP-70 cofactor; HSP24; Heat shock protein B25.3 

Gene Name

 grpE 

Gene Synonyms/Alias

 b2614; JW2594 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
7	*MSSKEQKTPEGQAP	acetylation	[1]
43	QVDPRDEKVANLEAQ	acetylation	[1]
43	QVDPRDEKVANLEAQ	pupylation	[2]
66	RDGILRVKAEMENLR	acetylation	[1]
66	RDGILRVKAEMENLR	pupylation	[2]
82	RTELDIEKAHKFALE	acetylation	[1]
85	LDIEKAHKFALEKFI	acetylation	[1, 3]
90	AHKFALEKFINELLP	acetylation	[3, 4]
111	RALEVADKANPDMSA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [4] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

 Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP- dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. 

Sequence Annotation

  

Keyword

 3D-structure; Chaperone; Complete proteome; Cytoplasm; Reference proteome; Stress response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 197 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0000774; F:adenyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0043335; P:protein unfolding; IDA:EcoCyc.
 GO:0009408; P:response to heat; IEP:EcoliWiki. 

Interpro

 IPR000740; GrpE.
 IPR013805; GrpE_coiled_coil.
 IPR009012; GrpE_head. 

Pfam

 PF01025; GrpE 

SMART

  

PROSITE

 PS01071; GRPE 

PRINTS

 PR00773; GRPEPROTEIN.