CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005962
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-like modifier-activating enzyme 1 Y 
Protein Synonyms/Alias
 Ubiquitin-activating enzyme E1; Ubiquitin-activating enzyme E1 Y 
Gene Name
 Uba1y 
Gene Synonyms/Alias
 Sby; Ube1ay; Ube1y; Ube1y1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
88GLGVEIAKNIILGGVacetylation[1]
184FCHSHGIKLVVADTRubiquitination[2]
469QEKLGKQKYFLVGAGacetylation[1]
851MYPIDFEKDDDSNFHubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. The Y chromosome form could be involved in the survival and proliferation of differentiating spermatogonia. 
Sequence Annotation
 ACT_SITE 631 631 Glycyl thioester intermediate (By  
Keyword
 ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1058 AA 
Protein Sequence
MSSSVLSKKR KVSGPDSSLD SSWSPTYSVM FGVPPGPTNE MSKNKEMDID ESLYSRQLYV 60
LGHEAMKHLQ ASSVLISGLQ GLGVEIAKNI ILGGVKAVTL HDQGIAQWAD LSSQFCLREE 120
DIGKNRAEIS QPRLAELNSY VPVFAYTGPL IEEFLSGFQV VVLTNTPLEY QLQVGEFCHS 180
HGIKLVVADT RGLVGQLFCD FGEEMILTDS NGEQPLSAMV SMITKENPGI VTCLEDSRHG 240
FESGDFISFT EVQGMSELNG IGPIEIKVLG PYTFSICDTS SFSEYIRGGI VSQVKVPRKI 300
NFKPLLASLA EPEFVVTDFA KCCHPAQLHI GFQALHQFCT QHSRPPRPHN EEDAEELVTL 360
AQSVNAQALP AVQQDCLDID LIRKLAYVAA GDLAPMNAFF GGLAAQEVMK ACSGKFMPIR 420
QWLYFDALEC LPEHRVAFME DKCLPHQNRY DGQVAVFGSD LQEKLGKQKY FLVGAGAIGC 480
ELLKNFAMIG LGCGEDGEIT VTDMDTIEKS NLNRQFLFRP WDITKLKSET AAAAVRDINP 540
HIRIFSHQNR VGPETEHVYD DDFFQKLDGV ANALDNVDAR LYVDRRCVYY RKPLLESGTL 600
GTKGNVQVVV PFLTESYSSS QDPPEKSIPI CTLKNFPNAI EHTVQWARDE FEGLFKQSAE 660
NVNQYLTDPK FMERTLQLAG TQPLEVLEAI HCSLVLQRPQ TWADCVTWAY QHWHTQYSHN 720
IQQLLHNFPP AQLTSSGALF WSGPKRCPHP LTFDINNPLH LDYVMAAANL FAQTYGLGGS 780
QDCAVVAKLL QSLPVPKFAP KSGIRIHVSE QELQSTSATT IDDSHLEELK TALPTPDKLL 840
GFKMYPIDFE KDDDSNFHMD FIVAASNLRA ENYGISPADR HKSKLIAGKI IPAIATTTSA 900
IVGLVCLELY KVVQGHQQLE SYKNSFINLA LPLFSFSAPL APECHQYYDQ EWTLWDRFDV 960
QGLQPSGEEM TLKQFLDYFK TEHKLEVIML SQGVSMLYSV FMPASKLKER LDQPMTEIVS 1020
CVSKQKLGHH VKSLVFELCC NSDSGDDIEV PYVRYIIR 1058 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008641; F:small protein activating enzyme activity; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 
Interpro
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR018965; Ub-activating_enz_e1_C.
 IPR023280; Ub-like_act_enz_cat_cys_dom.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018075; UBQ-activ_enz_E1.
 IPR018074; UBQ-activ_enz_E1_AS.
 IPR000011; UBQ/SUMO-activ_enz_E1-like. 
Pfam
 PF00899; ThiF
 PF09358; UBA_e1_C
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 
SMART
 SM00985; UBA_e1_C 
PROSITE
 PS00536; UBIQUITIN_ACTIVAT_1
 PS00865; UBIQUITIN_ACTIVAT_2 
PRINTS
 PR01849; UBIQUITINACT.