UniProt Accession

 ML12B_MOUSE; Q3THE2; Q3TVH2; Q9CQL8 

Genbank Protein ID

 AK002885; AK005133; AK146115; AK160123; AK168316; BC028878; BC099425; BC131925; BC131927 

Genbank Nucleotide ID

 BAB22432.1; BAB23832.1; BAE26912.1; BAE35646.1; BAE40255.1; AAH28878.1; AAH99425.1; AAI31926.1; AAI31928.1 

Protein Name

 Myosin regulatory light chain 12B 

Protein Synonyms/Alias

 Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; MLC20; Myosin regulatory light chain MRLC2 

Gene Name

 Myl12b 

Gene Synonyms/Alias

 Mrlc2; Mylc2b 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
51	NRDGFIDKEDLHDML	ubiquitination	[1]
150	YREAPIDKKGNFNYI	ubiquitination	[1]
151	REAPIDKKGNFNYIE	acetylation	[2]
151	REAPIDKKGNFNYIE	succinylation	[2]
151	REAPIDKKGNFNYIE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity). 

Sequence Annotation

 DOMAIN 29 64 EF-hand 1.
 DOMAIN 98 133 EF-hand 2.
 DOMAIN 134 169 EF-hand 3.
 MOD_RES 19 19 Phosphothreonine; by MLCK and ZIPK/DAPK3
 MOD_RES 20 20 Phosphoserine; by MLCK and ZIPK/DAPK3 (By  

Keyword

 Calcium; Complete proteome; Direct protein sequencing; Metal-binding; Motor protein; Muscle protein; Myosin; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 172 AA 

Protein Sequence

Gene Ontology

 GO:0045177; C:apical part of cell; IDA:MGI.
 GO:0016460; C:myosin II complex; IDA:MGI.
 GO:0001725; C:stress fiber; IDA:MGI.
 GO:0030018; C:Z disc; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0008360; P:regulation of cell shape; IMP:MGI. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 

Pfam

 PF13499; EF_hand_5 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS