CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029397
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 15 
Protein Synonyms/Alias
 TBC1 domain family, member 15, isoform CRA_d; cDNA FLJ77374 
Gene Name
 TBC1D15 
Gene Synonyms/Alias
 hCG_25175 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42SDAIPGLKINQQEEPubiquitination[1, 2, 3, 4]
78VSLEEWTKNIDSEGRubiquitination[1, 3, 4, 5]
93ILNVDNMKQMIFRGGubiquitination[1, 2, 3, 4, 5, 6, 7]
123YFPWDSTKEERTQLQubiquitination[1, 2, 3, 4, 7]
133RTQLQKQKTDEYFRMubiquitination[3, 5]
141TDEYFRMKLQWKSISubiquitination[3]
145FRMKLQWKSISQEQEubiquitination[3]
167DYRSLIEKDVNRTDRubiquitination[1, 3, 4]
334EKQQIMEKHYGFNEIubiquitination[6]
343YGFNEILKHINELSMubiquitination[4, 6]
370AISLQMVKCKELPQAubiquitination[3]
372SLQMVKCKELPQAVCubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 445 AA 
Protein Sequence
MIGFSKVTNY IFDSLRGSDP STHQRPPSEM ADFLSDAIPG LKINQQEEPG FEVITRIDLG 60
ERPVVQRREP VSLEEWTKNI DSEGRILNVD NMKQMIFRGG LSHALRKQAW KFLLGYFPWD 120
STKEERTQLQ KQKTDEYFRM KLQWKSISQE QEKRNSRLRD YRSLIEKDVN RTDRTNKFYE 180
GQDNPGLILL HDILMTYCMY DFDLGYVQGM SDLLSPLLYV MENEVDAFWC FASYMDQMHQ 240
NFEEQMQGMK TQLIQLSTLL RLLDSGFCSY LESQDSGYLY FCFRWLLIRF KREFSFLDIL 300
RLWEVMWTEL PCTNFHLLLC CAILESEKQQ IMEKHYGFNE ILKHINELSM KIDVEDILCK 360
AEAISLQMVK CKELPQAVCE ILGLQGSEVT TPDSDVGEDE NVVMTPCPTS AFQSNALPTL 420
SASGARNDSP TQIPVSSDVC RLTPA 445 
Gene Ontology
 GO:0005576; C:extracellular region; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005097; F:Rab GTPase activator activity; IEA:Compara. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS