CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002157
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA topoisomerase 1 
Protein Synonyms/Alias
 DNA topoisomerase I; Maintenance of killer protein 1 
Gene Name
 TOP1 
Gene Synonyms/Alias
 MAK1; YOL006C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
65KKKTKKIKTEPVQSSsumoylation[1]
91TSKPKKIKKEDGDVKsumoylation[1]
92SKPKKIKKEDGDVKVsumoylation[1]
Reference
 [1] Topoisomerase I-dependent viability loss in saccharomyces cerevisiae mutants defective in both SUMO conjugation and DNA repair.
 Chen XL, Silver HR, Xiong L, Belichenko I, Adegite C, Johnson ES.
 Genetics. 2007 Sep;177(1):17-30. [PMID: 17603101] 
Functional Description
 Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). 
Sequence Annotation
 REGION 357 358 Interaction with DNA (By similarity).
 REGION 420 425 Interaction with DNA (By similarity).
 REGION 512 514 Interaction with DNA (By similarity).
 ACT_SITE 727 727 O-(3'-phospho-DNA)-tyrosine intermediate.
 MOD_RES 14 14 Phosphoserine.
 MOD_RES 15 15 Phosphoserine.
 MOD_RES 24 24 Phosphoserine.
 MOD_RES 49 49 Phosphoserine.
 MOD_RES 76 76 Phosphoserine.
 CROSSLNK 65 65 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 91 91 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; ATP-binding; Complete proteome; DNA-binding; Isomerase; Isopeptide bond; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Topoisomerase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 769 AA 
Protein Sequence
MTIADASKVN HELSSDDDDD VPLSQTLKKR KVASMNSASL QDEAEPYDSD EAISKISKKK 60
TKKIKTEPVQ SSSLPSPPAK KSATSKPKKI KKEDGDVKVK TTKKEEQENE KKKREEEEEE 120
DKKAKEEEEE YKWWEKENED DTIKWVTLKH NGVIFPPPYQ PLPSHIKLYY DGKPVDLPPQ 180
AEEVAGFFAA LLESDHAKNP VFQKNFFNDF LQVLKESGGP LNGIEIKEFS RCDFTKMFDY 240
FQLQKEQKKQ LTSQEKKQIR LEREKFEEDY KFCELDGRRE QVGNFKVEPP DLFRGRGAHP 300
KTGKLKRRVN PEDIVLNLSK DAPVPPAPEG HKWGEIRHDN TVQWLAMWRE NIFNSFKYVR 360
LAANSSLKGQ SDYKKFEKAR QLKSYIDAIR RDYTRNLKSK VMLERQKAVA IYLIDVFALR 420
AGGEKSEDEA DTVGCCSLRY EHVTLKPPNT VIFDFLGKDS IRFYQEVEVD KQVFKNLTIF 480
KRPPKQPGHQ LFDRLDPSIL NKYLQNYMPG LTAKVFRTYN ASKTMQDQLD LIPNKGSVAE 540
KILKYNAANR TVAILCNHQR TVTKGHAQTV EKANNRIQEL EWQKIRCKRA ILQLDKDLLK 600
KEPKYFEEID DLTKEDEATI HKRIIDREIE KYQRKFVREN DKRKFEKEEL LPESQLKEWL 660
EKVDEKKQEF EKELKTGEVE LKSSWNSVEK IKAQVEKLEQ RIQTSSIQLK DKEENSQVSL 720
GTSKINYIDP RLSVVFCKKY DVPIEKIFTK TLREKFKWAI ESVDENWRF 769 
Gene Ontology
 GO:0005730; C:nucleolus; IPI:SGD.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0031298; C:replication fork protection complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0031490; F:chromatin DNA binding; IBA:RefGenome.
 GO:0003917; F:DNA topoisomerase type I activity; IDA:SGD.
 GO:0003918; F:DNA topoisomerase type II (ATP-hydrolyzing) activity; IEA:InterPro.
 GO:0006333; P:chromatin assembly or disassembly; IMP:SGD.
 GO:0000183; P:chromatin silencing at rDNA; IMP:SGD.
 GO:0006271; P:DNA strand elongation involved in DNA replication; IMP:SGD.
 GO:0006265; P:DNA topological change; IDA:SGD.
 GO:0007076; P:mitotic chromosome condensation; IGI:SGD.
 GO:0007097; P:nuclear migration; IMP:SGD.
 GO:0000019; P:regulation of mitotic recombination; IMP:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD. 
Interpro
 IPR011010; DNA_brk_join_enz.
 IPR013034; DNA_topo_domain1.
 IPR001631; TopoI.
 IPR018521; TopoI_AS.
 IPR025834; TopoI_C_dom.
 IPR014711; TopoI_cat_a-hlx-sub_euk.
 IPR014727; TopoI_cat_a/b-sub_euk.
 IPR013500; TopoI_cat_euk.
 IPR008336; TopoI_DNA-bd_euk.
 IPR013030; TopoI_DNA-bd_mixed-a/b_euk.
 IPR013499; TopoI_euk. 
Pfam
 PF14370; Topo_C_assoc
 PF01028; Topoisom_I
 PF02919; Topoisom_I_N 
SMART
 SM00435; TOPEUc 
PROSITE
 PS00176; TOPOISOMERASE_I_EUK 
PRINTS
 PR00416; EUTPISMRASEI.