UniProt Accession

 CHST7_HUMAN; Q9NS84; O75667 

Genbank Protein ID

 AB040711; AB037187; AL022165; BC045537 

Genbank Nucleotide ID

 BAB13770.1; BAB03217.1; CAA18154.1; AAH45537.1 

Protein Name

 Carbohydrate sulfotransferase 7 

Protein Synonyms/Alias

 Chondroitin 6-sulfotransferase 2; C6ST-2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5; GST-5; N-acetylglucosamine 6-O-sulfotransferase 4; GlcNAc6ST-4; Gn6st-4 

Gene Name

 CHST7 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
272	RDPGLNLKVVQLFRD	ubiquitination	[1, 2, 3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N- acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc. 

Sequence Annotation

 NP_BIND 110 116 PAPS (By similarity).
 NP_BIND 278 286 PAPS (By similarity).
 CARBOHYD 88 88 N-linked (GlcNAc...) (Potential).
 CARBOHYD 186 186 N-linked (GlcNAc...) (Potential).
 CARBOHYD 407 407 N-linked (GlcNAc...).  

Keyword

 Carbohydrate metabolism; Complete proteome; Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 486 AA 

Protein Sequence

Gene Ontology

 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0008459; F:chondroitin 6-sulfotransferase activity; IDA:UniProtKB.
 GO:0001517; F:N-acetylglucosamine 6-O-sulfotransferase activity; IDA:UniProtKB.
 GO:0030206; P:chondroitin sulfate biosynthetic process; IDA:UniProtKB.
 GO:0006044; P:N-acetylglucosamine metabolic process; IDA:UniProtKB.
 GO:0005976; P:polysaccharide metabolic process; TAS:UniProtKB. 

Interpro

 IPR016469; Carbohydrate_sulfotransferase.
 IPR027417; P-loop_NTPase.
 IPR000863; Sulfotransferase_dom. 

Pfam

 PF00685; Sulfotransfer_1 

SMART

  

PROSITE

  

PRINTS