CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001694
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methyl-CpG-binding domain protein 3 
Protein Synonyms/Alias
 Methyl-CpG-binding protein MBD3 
Gene Name
 MBD3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73TGKMLMSKMNKSRQRubiquitination[1]
90YDSSNQVKGKPDLNTubiquitination[1]
92SSNQVKGKPDLNTALubiquitination[1]
109RQTASIFKQPVTKITacetylation[2]
129KVKSDPQKAVDQPRQubiquitination[1]
141PRQLFWEKKLSGLNAacetylation[2]
141PRQLFWEKKLSGLNAubiquitination[1, 3, 4]
142RQLFWEKKLSGLNAFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. 
Sequence Annotation
 DOMAIN 1 72 MBD.
 MOD_RES 56 56 Phosphoserine.
 MOD_RES 85 85 Phosphoserine.
 MOD_RES 144 144 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 291 AA 
Protein Sequence
MERKRWECPA LPQGWEREEV PRRSGLSAGH RDVFYYSPSG KKFRSKPQLA RYLGGSMDLS 60
TFDFRTGKML MSKMNKSRQR VRYDSSNQVK GKPDLNTALP VRQTASIFKQ PVTKITNHPS 120
NKVKSDPQKA VDQPRQLFWE KKLSGLNAFD IAEELVKTMD LPKGLQGVGP GCTDETLLSA 180
IASALHTSTM PITGQLSAAV EKNPGVWLNT TQPLCKAFMV TDEDIRKQEE LVQQVRKRLE 240
EALMADMLAH VEELARDGEA PLDKACAEDD DEEDEEEEEE EPDPDPEMEH V 291 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000792; C:heterochromatin; IEA:Compara.
 GO:0016581; C:NuRD complex; NAS:BHF-UCL.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0016573; P:histone acetylation; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0006346; P:methylation-dependent chromatin silencing; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0009888; P:tissue development; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR016177; DNA-bd_integrase-typ.
 IPR025884; MeCpG-bd_2/3_C_dom.
 IPR001739; Methyl_CpG_DNA-bd. 
Pfam
 PF01429; MBD
 PF14048; MBD_C 
SMART
 SM00391; MBD 
PROSITE
 PS50982; MBD 
PRINTS