CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001298
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DnaJ homolog subfamily C member 8 
Protein Synonyms/Alias
 Splicing protein spf31 
Gene Name
 DNAJC8 
Gene Synonyms/Alias
 SPF31; HSPC315; HSPC331 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40RDSVLTSKNQIERLTubiquitination[1, 2]
105KAFEAVDKAYKLLLDubiquitination[1, 2]
108EAVDKAYKLLLDQEQubiquitination[3, 4]
127LDVIQAGKEYVEHTVubiquitination[2]
135EYVEHTVKERKKQLKacetylation[2]
135EYVEHTVKERKKQLKubiquitination[1]
146KQLKKEGKPTIVEEDacetylation[2, 5, 6, 7]
159EDDPELFKQAVYKQTubiquitination[1]
210KREREWQKNFEESRDubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
 DOMAIN 57 124 J.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 35 35 Phosphoserine.
 MOD_RES 146 146 N6-acetyllysine.  
Keyword
 Acetylation; Chaperone; Complete proteome; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 253 AA 
Protein Sequence
MAASGESGTS GGGGSTEEAF MTFYSEVKQI EKRDSVLTSK NQIERLTRPG SSYFNLNPFE 60
VLQIDPEVTD EEIKKRFRQL SILVHPDKNQ DDADRAQKAF EAVDKAYKLL LDQEQKKRAL 120
DVIQAGKEYV EHTVKERKKQ LKKEGKPTIV EEDDPELFKQ AVYKQTMKLF AELEIKRKER 180
EAKEMHERKR QREEEIEAQE KAKREREWQK NFEESRDGRV DSWRNFQANT KGKKEKKNRT 240
FLRPPKVKME QRE 253 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome. 
Interpro
 IPR001623; DnaJ_domain. 
Pfam
 PF00226; DnaJ 
SMART
 SM00271; DnaJ 
PROSITE
 PS00636; DNAJ_1
 PS50076; DNAJ_2 
PRINTS
 PR00625; JDOMAIN.