CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001232
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GTPase Era, mitochondrial 
Protein Synonyms/Alias
 H-ERA; hERA; Conserved ERA-like GTPase; CEGA; ERA-W; ERA-like protein 1 
Gene Name
 ERAL1 
Gene Synonyms/Alias
 HERA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
126LGAPNAGKSTLSNQLubiquitination[1, 2]
176PGIISPGKQKRHHLEubiquitination[2]
371VPYNVQQKTAVWEEGubiquitination[2, 3]
388GELVIQQKLLVPKESubiquitination[2]
393QQKLLVPKESYVKLLubiquitination[4, 5]
398VPKESYVKLLIGPKGubiquitination[4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly. 
Sequence Annotation
 DOMAIN 125 237 G.
 DOMAIN 360 437 KH type-2.
 NP_BIND 120 127 GTP (Potential).
 NP_BIND 167 171 GTP (Potential).
 NP_BIND 236 239 GTP (Potential).
 MOD_RES 173 173 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; GTP-binding; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Ribosome biogenesis; RNA-binding; rRNA-binding; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 437 AA 
Protein Sequence
MAAPSWRGAR LVQSVLRVWQ VGPHVARERV IPFSSLLGFQ RRCVSCVAGS AFSGPRLASA 60
SRSNGQGSAL DHFLGFSQPD SSVTPCVPAV SMNRDEQDVL LVHHPDMPEN SRVLRVVLLG 120
APNAGKSTLS NQLLGRKVFP VSRKVHTTRC QALGVITEKE TQVILLDTPG IISPGKQKRH 180
HLELSLLEDP WKSMESADLV VVLVDVSDKW TRNQLSPQLL RCLTKYSQIP SVLVMNKVDC 240
LKQKSVLLEL TAALTEGVVN GKKLKMRQAF HSHPGTHCPS PAVKDPNTQS VGNPQRIGWP 300
HFKEIFMLSA LSQEDVKTLK QYLLTQAQPG PWEYHSAVLT SQTPEEICAN IIREKLLEHL 360
PQEVPYNVQQ KTAVWEEGPG GELVIQQKLL VPKESYVKLL IGPKGHVISQ IAQEAGHDLM 420
DIFLCDVDIR LSVKLLK 437 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
 GO:0005525; F:GTP binding; NAS:UniProtKB.
 GO:0043024; F:ribosomal small subunit binding; IDA:UniProtKB.
 GO:0019843; F:rRNA binding; IDA:UniProtKB.
 GO:0000028; P:ribosomal small subunit assembly; IMP:UniProtKB. 
Interpro
 IPR005662; GTP-bd_Era.
 IPR006073; GTP_binding_domain.
 IPR015946; KH_dom-like_a/b.
 IPR009019; KH_prok-type.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom. 
Pfam
 PF01926; MMR_HSR1 
SMART
  
PROSITE
 PS50823; KH_TYPE_2 
PRINTS
 PR00326; GTP1OBG.