UniProt Accession

 NUBP1_HUMAN; P53384; Q32M30; Q498A9; Q53FS7 

Genbank Protein ID

 U01833; AK223204; BC100290; BC109322; BC109323 

Genbank Nucleotide ID

 AAA61932.1; BAD96924.1; AAI00291.1; AAI09323.1; AAI09324.1 

Protein Name

 Cytosolic Fe-S cluster assembly factor NUBP1 

Protein Synonyms/Alias

 Nucleotide-binding protein 1; NBP 1 

Gene Name

 NUBP1 

Gene Synonyms/Alias

 NBP; NBP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
47	DTAIEEIKEKMKTVK	ubiquitination	[1]
211	VSLQDVRKEINFCRK	ubiquitination	[2]
240	FICPKCKKESQIFPP	ubiquitination	[3]
275	PLDPLIGKNCDKGQS	ubiquitination	[2]
279	LIGKNCDKGQSFFID	ubiquitination	[2]
313	FCNLHQSKEENLISS	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Implicated in the regulation of centrosome duplication (By similarity). Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. 

Sequence Annotation

 NP_BIND 62 69 ATP (Potential).
 METAL 8 8 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 22 22 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 25 25 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 31 31 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 235 235 Iron-sulfur 2 (4Fe-4S); shared with
 METAL 238 238 Iron-sulfur 2 (4Fe-4S); shared with
 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 4Fe-4S; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Iron; Iron-sulfur; Metal-binding; Nucleotide-binding; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 320 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0051536; F:iron-sulfur cluster binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0000166; F:nucleotide binding; TAS:ProtInc.
 GO:0016049; P:cell growth; IMP:UniProtKB.
 GO:0006879; P:cellular iron ion homeostasis; IMP:UniProtKB.
 GO:0016226; P:iron-sulfur cluster assembly; IMP:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR025723; Anion-transp_ATPase-like_dom.
 IPR019591; ATPase-like_ParA/MinD.
 IPR000808; Mrp_CS.
 IPR027417; P-loop_NTPase. 

Pfam

 PF02374; ArsA_ATPase
 PF10609; ParA 

SMART

 SM00382; AAA 

PROSITE

 PS01215; MRP 

PRINTS