UniProt Accession

 ATG4C_HUMAN; Q96DT6; A6NLR8; D3DQ58; Q96K04 

Genbank Protein ID

 AJ312234; AJ320169; AK027773; AC103923; CH471059; CH471059; BC033024 

Genbank Nucleotide ID

 CAC85556.1; CAC43939.1; BAB55356.1; EAX06579.1; EAX06580.1; AAH33024.1 

Protein Name

 Cysteine protease ATG4C 

Protein Synonyms/Alias

 AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C 

Gene Name

 ATG4C 

Gene Synonyms/Alias

 APG4C; AUTL1; AUTL3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	TGTDEVDKLKTKFIS	ubiquitination	[1]
15	EVDKLKTKFISAWNN	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. 

Sequence Annotation

 ACT_SITE 111 111 Nucleophile (By similarity).
 ACT_SITE 345 345 Potential.
 ACT_SITE 347 347 By similarity.
 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 Acetylation; Autophagy; Complete proteome; Cytoplasm; Hydrolase; Protease; Protein transport; Reference proteome; Thiol protease; Transport; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 458 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005576; C:extracellular region; IDA:UniProtKB.
 GO:0004197; F:cysteine-type endopeptidase activity; IMP:UniProtKB.
 GO:0000045; P:autophagic vacuole assembly; IGI:UniProtKB.
 GO:0006612; P:protein targeting to membrane; ISS:UniProtKB.
 GO:0006508; P:proteolysis; IDA:UniProtKB. 

Interpro

 IPR005078; Peptidase_C54. 

Pfam

 PF03416; Peptidase_C54 

SMART

  

PROSITE

  

PRINTS