CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010769
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional activator protein Pur-alpha 
Protein Synonyms/Alias
 Purine-rich single-stranded DNA-binding protein alpha 
Gene Name
 PURA 
Gene Synonyms/Alias
 PUR1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87NAKGRFLKIAEVGAGubiquitination[1]
97EVGAGGNKSRLTLSMubiquitination[1]
144DEPRRALKSEFLVREubiquitination[1, 2]
229TSLTVDNKRFFFDVGubiquitination[1]
239FFDVGSNKYGVFMRVubiquitination[2, 3, 4]
250FMRVSEVKPTYRNSIubiquitination[1, 5]
262NSITVPYKVWAKFGHubiquitination[1, 2, 3, 4, 5]
266VPYKVWAKFGHTFCKubiquitination[1, 5]
273KFGHTFCKYSEEMKKacetylation[6]
273KFGHTFCKYSEEMKKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. 
Sequence Annotation
 MOD_RES 253 253 Phosphotyrosine (By similarity).  
Keyword
 Activator; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 322 AA 
Protein Sequence
MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGGA PGGLQHETQE 60
LASKRVDIQN KRFYLDVKQN AKGRFLKIAE VGAGGNKSRL TLSMSVAVEF RDYLGDFIEH 120
YAQLGPSQPP DLAQAQDEPR RALKSEFLVR ENRKYYMDLK ENQRGRFLRI RQTVNRGPGL 180
GSTQGQTIAL PAQGLIEFRD ALAKLIDDYG VEEEPAELPE GTSLTVDNKR FFFDVGSNKY 240
GVFMRVSEVK PTYRNSITVP YKVWAKFGHT FCKYSEEMKK IQEKQREKRA ACEQLHQQQQ 300
QQQEETAAAT LLLQGEEEGE ED 322 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0005662; C:DNA replication factor A complex; ISS:UniProtKB.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0000784; C:nuclear chromosome, telomeric region; IC:UniProtKB.
 GO:0003691; F:double-stranded telomeric DNA binding; IDA:UniProtKB.
 GO:0032422; F:purine-rich negative regulatory element binding; IEA:Compara.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
 GO:0008134; F:transcription factor binding; ISS:UniProtKB.
 GO:0000900; F:translation repressor activity, nucleic acid binding; IEA:Compara.
 GO:0006270; P:DNA replication initiation; TAS:ProtInc.
 GO:0006268; P:DNA unwinding involved in replication; IDA:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0007399; P:nervous system development; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara. 
Interpro
 IPR006628; PUR_DNA_RNA-bd. 
Pfam
 PF04845; PurA 
SMART
 SM00712; PUR 
PROSITE
  
PRINTS