UniProt Accession

 CDK8_HUMAN; P49336; Q5VUF3; Q6ISB5 

Genbank Protein ID

 X85753; AL590108; AL159159; AL159159; AL590108; CH471075; CH471075; BC069634; BC104492; BC105950; BC107601 

Genbank Nucleotide ID

 CAA59754.1; CAH72300.1; CAH72300.1; CAI16960.1; CAI16960.1; EAX08386.1; EAX08387.1; AAH69634.1; AAI04493.1; AAI05951.1; AAI07602.1 

Protein Name

 Cyclin-dependent kinase 8 

Protein Synonyms/Alias

 Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35 

Gene Name

 CDK8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
367	TEEEPDDKGDKKNQQ	acetylation	[1]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. 

Sequence Annotation

 DOMAIN 21 335 Protein kinase.
 NP_BIND 27 35 ATP (By similarity).
 REGION 1 15 Interaction with CCNC.
 ACT_SITE 151 151 Proton acceptor (By similarity).
 BINDING 52 52 ATP (By similarity).  

Keyword

 3D-structure; Activator; Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Repressor; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 464 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IEA:EC.
 GO:0004672; F:protein kinase activity; TAS:ProtInc.
 GO:0008353; F:RNA polymerase II carboxy-terminal domain kinase activity; IEA:EC.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS