CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029089
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 ADP-sugar pyrophosphatase 
Protein Synonyms/Alias
  
Gene Name
 NUDT5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14TESSQNGKQYIISEEubiquitination[1]
30ISEGKWVKLEKTTYMubiquitination[1]
33GKWVKLEKTTYMDPTubiquitination[1]
42TYMDPTGKTRRNKEKubiquitination[1]
63LRTWESVKRTTRKEQubiquitination[1]
68SVKRTTRKEQTADGVubiquitination[1]
188VEVISLPKNDLLQRLubiquitination[2, 3, 4, 5, 6]
218YSYALALKHANAKPFubiquitination[7]
223ALKHANAKPFEVPFLubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
231PFEVPFLKF******ubiquitination[1, 2, 3, 4, 5, 7, 8, 9]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 232 AA 
Protein Sequence
MESQEPTESS QNGKQYIISE ELISEGKWVK LEKTTYMDPT GKTRRNKEKT TTLLILRTWE 60
SVKRTTRKEQ TADGVAVIPV LQRTLHYECI VLVKQFRPPM GGYCIEFPAG LIDDGETPEA 120
AALRELEEET GYKGDIAECS PAVCMDPGLS NCTIHIVTVT INGDDAENAR PKPKPGDGEF 180
VEVISLPKND LLQRLDALVA EEHLTVDARV YSYALALKHA NAKPFEVPFL KF 232 
Gene Ontology
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. 
Interpro
 IPR020476; Nudix_hydrolase.
 IPR020084; NUDIX_hydrolase_CS.
 IPR000086; NUDIX_hydrolase_dom.
 IPR015797; NUDIX_hydrolase_dom-like. 
Pfam
 PF00293; NUDIX 
SMART
  
PROSITE
 PS51462; NUDIX
 PS00893; NUDIX_BOX 
PRINTS
 PR00502; NUDIXFAMILY.