CPLM-007794

Genbank Nucleotide ID

Glyceraldehyde-3-phosphate dehydrogenase

Protein Synonyms/Alias

GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

Oryctolagus cuniculus (Rabbit)

Position	Peptide	Type	References
184	HAITATQKTVDGPSG	acetylation	[1, 2, 3]

[1] Role of lysine-183 in D-glyceraldehyde-3-phosphate dehydrogenases. Properties of the N-acetylated yeast, sturgeon muscle and rabbit muscle enzymes.
Foucault G, Nakano M, Pudles J.
Eur J Biochem. 1978 Feb 1;83(1):113-23. [PMID: 342241]
[2] Enzymatic characterization of the N-acetylation of 3-phosphoglyceraldehyde dehydrogenase by acetyl phosphate.
Park JH, Shaw DC, Mathew E, Meriwether BP.
J Biol Chem. 1970 Jun 10;245(11):2946-53. [PMID: 4316369]
[3] N-ACETYLATION OF 3-PHOSPHOGLYCERALDEHYDE DEHYDROGENASE BY SUBSTRATES.
MATHEW E, AGNELLO CF, PARK JH.
J Biol Chem. 1965 Jul;240:PC3232-5. [PMID: 14342361]

Functional Description

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity).

NP_BIND 11 12 NAD.
REGION 2 146 Interaction with WARS (By similarity).
REGION 149 151 Glyceraldehyde 3-phosphate binding (By
REGION 209 210 Glyceraldehyde 3-phosphate binding (By
ACT_SITE 150 150 Nucleophile.
BINDING 33 33 NAD.
BINDING 78 78 NAD; via carbonyl oxygen.
BINDING 120 120 NAD (By similarity).
BINDING 180 180 Glyceraldehyde 3-phosphate (By
BINDING 232 232 Glyceraldehyde 3-phosphate (By
BINDING 314 314 NAD.
MOD_RES 3 3 N6,N6-dimethyllysine (By similarity).
MOD_RES 7 7 Deamidated asparagine (By similarity).
MOD_RES 40 40 Phosphotyrosine (By similarity).
MOD_RES 59 59 N6-acetyllysine (By similarity).
MOD_RES 62 62 Deamidated asparagine (By similarity).
MOD_RES 64 64 N6,N6-dimethyllysine (By similarity).
MOD_RES 68 68 Deamidated asparagine (By similarity).
MOD_RES 73 73 Phosphothreonine (By similarity).
MOD_RES 120 120 Phosphoserine (By similarity).
MOD_RES 146 146 Phosphoserine (By similarity).
MOD_RES 147 147 Deamidated asparagine (By similarity).
MOD_RES 149 149 Phosphoserine (By similarity).
MOD_RES 150 150 ADP-ribosylcysteine; by autocatalysis; in
MOD_RES 150 150 Cysteine persulfide (By similarity).
MOD_RES 150 150 S-nitrosocysteine; in reversibly
MOD_RES 153 153 Deamidated asparagine (By similarity).
MOD_RES 182 182 Phosphothreonine (By similarity).
MOD_RES 192 192 N6,N6-dimethyllysine; alternate (By
MOD_RES 192 192 N6-acetyllysine; alternate (By
MOD_RES 192 192 N6-malonyllysine; alternate (By
MOD_RES 209 209 Phosphothreonine (By similarity).
MOD_RES 213 213 N6,N6-dimethyllysine; alternate (By
MOD_RES 213 213 N6-malonyllysine; alternate (By
MOD_RES 217 217 N6-acetyllysine (By similarity).
MOD_RES 223 223 Deamidated asparagine (By similarity).
MOD_RES 225 225 N6,N6-dimethyllysine; alternate (By
MOD_RES 225 225 N6-acetyllysine; alternate (By
MOD_RES 227 227 Phosphothreonine (By similarity).
MOD_RES 235 235 Phosphothreonine (By similarity).
MOD_RES 252 252 N6-acetyllysine (By similarity).
MOD_RES 258 258 N6,N6-dimethyllysine (By similarity).
MOD_RES 261 261 N6,N6-dimethyllysine (By similarity).
MOD_RES 310 310 Phosphoserine (By similarity).
MOD_RES 312 312 Phosphotyrosine (By similarity).
MOD_RES 314 314 Deamidated asparagine (By similarity).
MOD_RES 318 318 Phosphotyrosine (By similarity).
MOD_RES 332 332 N6,N6-dimethyllysine (By similarity).

3D-structure; Acetylation; ADP-ribosylation; Apoptosis; Complete proteome; Cytoplasm; Cytoskeleton; Glycolysis; Methylation; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; S-nitrosylation; Transferase; Translation regulation; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; ISS:UniProtKB.
GO:0015630; C:microtubule cytoskeleton; ISS:UniProtKB.
GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; ISS:UniProtKB.
GO:0008017; F:microtubule binding; ISS:UniProtKB.
GO:0051287; F:NAD binding; IEA:InterPro.
GO:0050661; F:NADP binding; IEA:InterPro.
GO:0035605; F:peptidyl-cysteine S-nitrosylase activity; ISS:UniProtKB.
GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
GO:0000226; P:microtubule cytoskeleton organization; ISS:UniProtKB.
GO:0051402; P:neuron apoptotic process; ISS:UniProtKB.
GO:0035606; P:peptidyl-cysteine S-trans-nitrosylation; ISS:UniProtKB.
GO:0050821; P:protein stabilization; ISS:UniProtKB.
GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.

IPR020831; GlycerAld/Erythrose_P_DH.
IPR020830; GlycerAld_3-P_DH_AS.
IPR020829; GlycerAld_3-P_DH_cat.
IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424; Glyceraldehyde-3-P_DH_1.
IPR016040; NAD(P)-bd_dom.

PF02800; Gp_dh_C
PF00044; Gp_dh_N

PR00078; G3PDHDRGNASE.