CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012713
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gamma-interferon-inducible protein 16 
Protein Synonyms/Alias
 Ifi-16; Interferon-inducible myeloid differentiation transcriptional activator 
Gene Name
 IFI16 
Gene Synonyms/Alias
 IFNGIP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
443PSSSFFTKLKPRLKTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. 
Sequence Annotation
 DOMAIN 1 88 DAPIN.
 DOMAIN 189 389 HIN-200 1.
 DOMAIN 562 761 HIN-200 2.
 REGION 192 393 Interaction with TP53 C-terminus.
 REGION 571 766 Interaction with TP53 core domain.
 MOTIF 96 100 Nuclear localization signal.
 MOTIF 128 131 Nuclear localization signal.
 MOTIF 134 136 Nuclear localization signal.
 MOTIF 140 143 Nuclear localization signal.
 MOD_RES 95 95 Phosphoserine.
 MOD_RES 99 99 N6-acetyllysine.
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 153 153 Phosphoserine.
 MOD_RES 174 174 Phosphoserine.
 MOD_RES 214 214 N6-acetyllysine.
 MOD_RES 444 444 N6-acetyllysine.
 MOD_RES 451 451 N6-acetyllysine.
 MOD_RES 561 561 N6-acetyllysine; alternate.
 MOD_RES 575 575 Phosphoserine.
 MOD_RES 598 598 N6-acetyllysine.
 MOD_RES 614 614 N6-acetyllysine.
 MOD_RES 780 780 Phosphoserine.
 CROSSLNK 561 561 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Apoptosis; Autophagy; Complete proteome; Cytoplasm; DNA-binding; Immunity; Inflammatory response; Innate immunity; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 785 AA 
Protein Sequence
MGKKYKNIVL LKGLEVINDY HFRMVKSLLS NDLKLNLKMR EEYDKIQIAD LMEEKFRGDA 60
GLGKLIKIFE DIPTLEDLAE TLKKEKLKVK GPALSRKRKK EVDATSPAPS TSSTVKTEGA 120
EATPGAQKRK KSTKEKAGPK GSKVSEEQTQ PPSPAGAGMS TAMGRSPSPK TSLSAPPNSS 180
STENPKTVAK CQVTPRRNVL QKRPVIVKVL STTKPFEYET PEMEKKIMFH ATVATQTQFF 240
HVKVLNTSLK EKFNGKKIII ISDYLEYDSL LEVNEESTVS EAGPNQTFEV PNKIINRAKE 300
TLKIDILHKQ ASGNIVYGVF MLHKKTVNQK TTIYEIQDDR GKMDVVGTGQ CHNIPCEEGD 360
KLQLFCFRLR KKNQMSKLIS EMHSFIQIKK KTNPRNNDPK SMKLPQEQRQ LPYPSEASTT 420
FPESHLRTPQ MPPTTPSSSF FTKLKPRLKT EPEEVSIEDS AQSDLKEVMV LNATESFVYE 480
PKEQKKMFHA TVATENEVFR VKVFNIDLKE KFTPKKIIAI ANYVCRNGFL EVYPFTLVAD 540
VNADRNMEIP KGLIRSASVT PKINQLCSQT KGSFVNGVFE VHKKNVRGEF TYYEIQDNTG 600
KMEVVVHGRL TTINCEEGDK LKLTCFELAP KSGNTGELRS VIHSHIKVIK TRKNKKDILN 660
PDSSMETSPD FFF 673 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016607; C:nuclear speck; IDA:MGI.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0003690; F:double-stranded DNA binding; IDA:UniProtKB.
 GO:0008134; F:transcription factor binding; IDA:UniProtKB.
 GO:0097202; P:activation of cysteine-type endopeptidase activity; IMP:UniProtKB.
 GO:0002218; P:activation of innate immune response; IDA:UniProtKB.
 GO:0006914; P:autophagy; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; NAS:UniProtKB.
 GO:0042149; P:cellular response to glucose starvation; IDA:UniProtKB.
 GO:0071479; P:cellular response to ionizing radiation; IDA:UniProtKB.
 GO:0051607; P:defense response to virus; IMP:UniProtKB.
 GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; IDA:MGI.
 GO:0030224; P:monocyte differentiation; IDA:UniProtKB.
 GO:2000117; P:negative regulation of cysteine-type endopeptidase activity; IDA:UniProtKB.
 GO:0043392; P:negative regulation of DNA binding; IDA:UniProtKB.
 GO:0045824; P:negative regulation of innate immune response; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0045071; P:negative regulation of viral genome replication; IDA:UniProtKB.
 GO:0032731; P:positive regulation of interleukin-1 beta production; IDA:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0032481; P:positive regulation of type I interferon production; TAS:Reactome.
 GO:0010506; P:regulation of autophagy; IEP:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR004020; DAPIN.
 IPR011029; DEATH-like_dom.
 IPR004021; HIN200/IF120x.
 IPR012340; NA-bd_OB-fold. 
Pfam
 PF02760; HIN
 PF02758; PYRIN 
SMART
  
PROSITE
 PS50824; DAPIN
 PS50834; HIN_200 
PRINTS