CPLM-012413

Genbank Nucleotide ID

Nucleolar and coiled-body phosphoprotein 1

Protein Synonyms/Alias

140 kDa nucleolar phosphoprotein; Nopp140; Hepatitis C virus NS5A-transactivated protein 13; HCV NS5A-transactivated protein 13; Nucleolar 130 kDa protein; Nucleolar phosphoprotein p130

Homo sapiens (Human)

Position	Peptide	Type	References
33	QLSEVANKFAKATGA	acetylation	[1, 2, 3, 4, 5]
33	QLSEVANKFAKATGA	ubiquitination	[5, 6]
36	EVANKFAKATGATQQ	acetylation	[2]
76	QANGPVAKKAKKKAS	acetylation	[5]
80	PVAKKAKKKASSSDS	methylation	[7]
193	KITPVTVKAQTKAPP	ubiquitination	[5]
197	VTVKAQTKAPPKPAR	ubiquitination	[5]
390	TTKNSSNKPAVTTKS	acetylation	[5]
401	TTKSPAVKPAAAPKQ	acetylation	[5]
415	QPVGGGQKLLTRKAD	acetylation	[1, 4, 5, 8]
446	KKMVATTKPKATAKA	acetylation	[5]
505	AGGAAPSKPASAKKG	acetylation	[5]
510	PSKPASAKKGKAESS	acetylation	[5]
601	EAETPQAKKIKLQTP	acetylation	[5]
647	ADNSFDAKRGAAGDW	acetylation	[5]
647	ADNSFDAKRGAAGDW	ubiquitination	[9, 10]
663	ERANQVLKFTKGKSF	acetylation	[1]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
[3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[7] Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome.
Bremang M, Cuomo A, Agresta AM, Stugiewicz M, Spadotto V, Bonaldi T.
Mol Biosyst. 2013 Jul 30;9(9):2231-47. [PMID: 23748837]
[8] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[9] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[10] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.

DOMAIN 10 42 LisH.
REPEAT 84 95 Acidic serine cluster 1.
REPEAT 125 136 Acidic serine cluster 2.
REPEAT 167 178 Acidic serine cluster 3.
REPEAT 221 232 Acidic serine cluster 4.
REPEAT 264 275 Acidic serine cluster 5.
REPEAT 325 336 Acidic serine cluster 6.
REPEAT 363 375 Acidic serine cluster 7.
REPEAT 425 436 Acidic serine cluster 8.
REPEAT 470 481 Acidic serine cluster 9.
REPEAT 519 529 Acidic serine cluster 10.
REPEAT 555 566 Acidic serine cluster 11.
REGION 84 566 11 X 12 AA approximate repeats of an
REGION 204 382 Interacts with RPA194.
MOTIF 68 82 Nuclear localization signal (Potential).
MOTIF 384 587 Nuclear localization signal (Potential).
MOTIF 601 617 Nuclear localization signal (Potential).
MOD_RES 33 33 N6-acetyllysine.
MOD_RES 87 87 Phosphoserine.
MOD_RES 90 90 Phosphoserine.
MOD_RES 91 91 Phosphoserine.
MOD_RES 366 366 Phosphoserine.
MOD_RES 397 397 Phosphoserine.
MOD_RES 415 415 N6-acetyllysine.
MOD_RES 508 508 Phosphoserine.
MOD_RES 538 538 Phosphoserine.
MOD_RES 563 563 Phosphoserine.
MOD_RES 580 580 Phosphoserine.
MOD_RES 582 582 Phosphoserine.
MOD_RES 607 607 Phosphothreonine.
MOD_RES 610 610 Phosphothreonine.
MOD_RES 622 622 Phosphoserine.
MOD_RES 643 643 Phosphoserine.
MOD_RES 663 663 N6-acetyllysine.
MOD_RES 686 686 Phosphoserine.
MOD_RES 698 698 Phosphoserine.

Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; GTP-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0015030; C:Cajal body; IEA:Compara.
GO:0005737; C:cytoplasm; IDA:HPA.
GO:0005730; C:nucleolus; IDA:HPA.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
GO:0007067; P:mitosis; TAS:ProtInc.
GO:0007000; P:nucleolus organization; IEA:Compara.
GO:0006364; P:rRNA processing; TAS:ProtInc.

IPR006594; LisH_dimerisation.
IPR007718; SRP40_C.