CPLM-009331

Genbank Nucleotide ID

Ras-related protein Rap-1b

Protein Synonyms/Alias

GTP-binding protein smg p21B

Homo sapiens (Human)

Position	Peptide	Type	References
5	***MREYKLVVLGSG	ubiquitination	[1]
31	VQGIFVEKYDPTIED	ubiquitination	[2, 3, 4, 5]
117	PMILVGNKCDLEDER	ubiquitination	[6]
128	EDERVVGKEQGQNLA	ubiquitination	[6]
151	LESSAKSKINVNEIF	ubiquitination	[6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction.

NP_BIND 10 18 GTP.
NP_BIND 57 61 GTP.
NP_BIND 116 119 GTP.
NP_BIND 147 149 GTP.
REGION 25 67 Interaction with KRIT1.
MOTIF 32 40 Effector region (Probable).
MOD_RES 39 39 ADP-ribosylserine; by botulinum toxin
MOD_RES 179 179 Phosphoserine; by PKA.
MOD_RES 181 181 Cysteine methyl ester.
LIPID 181 181 S-geranylgeranyl cysteine.

3D-structure; ADP-ribosylation; Alternative splicing; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Phosphoprotein; Prenylation; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005911; C:cell-cell junction; IDA:UniProtKB.
GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
GO:0005811; C:lipid particle; IDA:UniProtKB.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0019003; F:GDP binding; IDA:UniProtKB.
GO:0005525; F:GTP binding; IDA:UniProtKB.
GO:0003924; F:GTPase activity; IDA:UniProtKB.
GO:0008283; P:cell proliferation; IEA:Compara.
GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
GO:0030168; P:platelet activation; TAS:Reactome.
GO:2000114; P:regulation of establishment of cell polarity; IMP:UniProtKB.
GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
GO:0007264; P:small GTPase mediated signal transduction; IEA:Compara.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR020849; Small_GTPase_Ras.

PR00449; RASTRNSFRMNG.