| Tag | Content |
|---|
| CPLM ID | CPLM-015727 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase |
Protein Synonyms/Alias | Peptide:N-glycanase |
Gene Name | Pngl |
Gene Synonyms/Alias | PNGase; CG7865 |
Created Date | July 27, 2013 |
Organism | Drosophila melanogaster (Fruit fly) |
NCBI Taxa ID | 7227 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 517 | QKAQFSSKNIFRKVE | acetylation | [1] |
|
Reference | [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation. Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C. Sci Signal. 2011 Jul 26;4(183):ra48. [ PMID: 21791702] |
Functional Description | Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity). |
Sequence Annotation | DOMAIN 34 97 PUB.
DOMAIN 441 631 PAW.
ACT_SITE 296 296 Nucleophile (By similarity).
ACT_SITE 323 323 By similarity.
ACT_SITE 340 340 By similarity.
METAL 246 246 Zinc (By similarity).
METAL 249 249 Zinc (By similarity).
METAL 272 272 Zinc (By similarity).
METAL 273 273 Zinc (By similarity).
MOD_RES 126 126 Phosphoserine. |
Keyword | Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Phosphoprotein; Reference proteome; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 631 AA |
Protein Sequence | MVDINLECVH QIEPKTRSAG QQQQERGLKE AYLEAVRILL VLLENILAQP ENSMFRTIRQ 60
ENKAIKEKLL SLPGCERLLE AIGFVRAPSS NAYTLPTEVS LQQVKKYRDA LSERRTAWLN 120
GTVSKSPPQQ STTSTTPLFI KPSVEYRHRI AFPRVLRTNN NFLQSLELYS DAVMQYEDNL 180
LLATGRTLIP VEELTEMASE KLIDIQDQIA SGERQEKEPC VRDLLLVELV NWFNTQFFQW 240
VNNIPCRVCG SEESRLRRTE REGDIRVEVT VCCGQESKFY RYNDISQLLV SRKGRCGEYA 300
NCFTFLCRAL DYDARIVHSH FDHVWTEVYS EAQMRWLHVD PSENVIDSPL MYQHGWKRHI 360
DYILAYSRDD IQDVTWRYTN DHQKILHLRK LCGEKEMVQT LDAIRAKRRQ NCTADRKLFL 420
SQRNMYEVIG LTLERKPTEN ELKGRSSGSL SWRQSRGEHT FTNIFVFNLS ATELQKRQLN 480
VRYSCATDTY ERYAKEGEHI TILDSYKTWQ KAQFSSKNIF RKVERDWKMA YLARLEDTDC 540
GEIAWTFDFS KTNLKVKSYN LVFETKTFGD GKISVTVDAT DGSASVENAT GFKIVAKLTG 600
GKGDVAWQHT QLFRQSLNSR DYPFDLQVQL H 631 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |