| Tag | Content |
|---|
| CPLM ID | CPLM-024312 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | E3 ubiquitin-protein ligase SMURF2 |
Protein Synonyms/Alias | SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2 |
Gene Name | Smurf2 |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 345 | LNRQNQLKDQQQQQV | ubiquitination | [1] | | 615 | VIPQHLLKTFDEKEL | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level (By similarity). |
Sequence Annotation | DOMAIN 1 98 C2.
DOMAIN 157 190 WW 1.
DOMAIN 251 284 WW 2.
DOMAIN 297 330 WW 3.
DOMAIN 414 748 HECT.
ACT_SITE 716 716 Glycyl thioester intermediate (By |
Keyword | Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Ligase; Membrane; Nucleus; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 748 AA |
Protein Sequence | MSNPGGRRNG PVKLRLTVLC AKNLVKKDFF RLPDPFAKVV VDGSGQCHST DTVKNTLDPK 60
WNQHYDLYIG KSDSVTISVW NHKKIHKKQG AGFLGCVRLL SNAINRLKDT GYQRLDLCKL 120
GPNDNDTVRG QIVVSLQSRD RIGTGGQVVD CSRLFDNDLP DGWEERRTAS GRIQYLNHIT 180
RTTQWERPTR PASEYSSPGR PLSCFVDENT PITGTNGATC GHSSDPRLAE RRVRSQRHRN 240
YMSRTHLHTP PDLPEGYEQR TTQQGQVYFL HTQTGVSTWH DPRVPRDLSN INCEELGPLP 300
PGWEIRNTAT GRVYFVDHNN RTTQFTDPRL SANLHLVLNR QNQLKDQQQQ QVVPLCPDDT 360
ECLTVPRYKR DLVQKLKILR QELSQQQPQA GHCRIEVSRE EIFEESYRQV MKMRPKDLWK 420
RLMIKFRGEE GLDYGGVARE WLYLLSHEML NPYYGLFQYS RDDIYTLQIN PDSAVNPEHL 480
SYFHFVGRIM GMAVFHGHYI DGGFTLPFYK QLLGKSITLD DMELVDPDLH NSLVWILEND 540
ITGVLDHTFC VEHNAYGEII QHELKPNGKS IPVTEENKKE YVRLYVNWRF LRGIEAQFLA 600
LQKGFNEVIP QHLLKTFDEK ELELIICGLG KIDVSDWKVN TRLKHCTPDS NVVKWFWKAV 660
EFFDEERRAR LLQFVTGSSR VPLQGFKALQ GAAGPRLFTI HQIDACTNNL PKAHTCFNRI 720
DIPPYESYEK LYEKLLTAIE ETCGFAVE 748 |
Gene Ontology | GO:0005737; C:cytoplasm; IBA:RefGenome. GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell. GO:0005634; C:nucleus; IBA:RefGenome. GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. GO:0000151; C:ubiquitin ligase complex; IEA:Compara. GO:0004842; F:ubiquitin-protein ligase activity; IBA:RefGenome. GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. GO:0007179; P:transforming growth factor beta receptor signaling pathway; IBA:RefGenome. GO:0030579; P:ubiquitin-dependent SMAD protein catabolic process; IEA:Compara. |
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PROSITE | |
PRINTS | |