CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023009
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methyl-CpG-binding domain protein 1 
Protein Synonyms/Alias
 CXXC-type zinc finger protein 3; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1 
Gene Name
 MBD1 
Gene Synonyms/Alias
 CXXC3; PCM1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
422HHLGPTLKPTLATRTacetylation[1]
422HHLGPTLKPTLATRTubiquitination[2]
499VVALPQVKQEKADTQsumoylation[3]
538DPGLPSVKQEPPDPEsumoylation[3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins.
 Lyst MJ, Nan X, Stancheva I.
 EMBO J. 2006 Nov 15;25(22):5317-28. [PMID: 17066076] 
Functional Description
 Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. 
Sequence Annotation
 DOMAIN 1 69 MBD.
 ZN_FING 169 216 CXXC-type 1.
 ZN_FING 217 263 CXXC-type 2.
 ZN_FING 330 378 CXXC-type 3.
 REGION 529 592 TRD.
 MOTIF 84 88 Nuclear localization signal (Potential).
 MOD_RES 399 399 Phosphoserine.
 CROSSLNK 499 499 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 538 538 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; Chromosome; Complete proteome; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 605 AA 
Protein Sequence
MAEDWLDCPA LGPGWKRREV FRKSGATCGR SDTYYQSPTG DRIRSKVELT RYLGPACDLT 60
LFDFKQGILC YPAPKAHPVA VASKKRKKPS RPAKTRKRQV GPQSGEVRKE APRDETKADT 120
DTAPASFPAP GCCENCGISF SGDGTQRQRL KTLCKDCRAQ RIAFNREQRM FKRVGCGECA 180
ACQVTEDCGA CSTCLLQLPH DVASGLFCKC ERRRCLRIVE RSRGCGVCRG CQTQEDCGHC 240
PICLRPPRPG LRRQWKCVQR RCLRGKHARR KGGCDSKMAA RRRPGAQPLP PPPPSQSPEP 300
TEPHPRALAP SPPAEFIYYC VDEDELQPYT NRRQNRKCGA CAACLRRMDC GRCDFCCDKP 360
KFGGSNQKRQ KCRWRQCLQF AMKRLLPSVW SESEDGAGSP PPYRRRKRPS SARRHHLGPT 420
LKPTLATRTA QPDHTQAPTK QEAGGGFVLP PPGTDLVFLR EGASSPVQVP GPVAASTEAL 480
LQEAQCSGLS WVVALPQVKQ EKADTQDEWT PGTAVLTSPV LVPGCPSKAV DPGLPSVKQE 540
PPDPEEDKEE NKDDSASKLA PEEEAGGAGT PVITEIFSLG GTRFRDTAVW LPRSKDLKKP 600
GARKQ 605 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000792; C:heterochromatin; IEA:Compara.
 GO:0016363; C:nuclear matrix; ISS:UniProtKB.
 GO:0016607; C:nuclear speck; ISS:UniProtKB.
 GO:0008327; F:methyl-CpG binding; NAS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR016177; DNA-bd_integrase-typ.
 IPR001739; Methyl_CpG_DNA-bd.
 IPR002857; Znf_CXXC. 
Pfam
 PF01429; MBD
 PF02008; zf-CXXC 
SMART
 SM00391; MBD 
PROSITE
 PS50982; MBD
 PS51058; ZF_CXXC 
PRINTS