CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031505
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Galactokinase 
Protein Synonyms/Alias
 Galactokinase 1; cDNA FLJ56840, highly similar to Galactokinase (EC 2.7.1.6) 
Gene Name
 GALK1 
Gene Synonyms/Alias
 hCG_27540 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99VLVGSPRKDGLVSLLubiquitination[1, 2, 3]
247LVPLSDPKLAVLITNubiquitination[1, 3]
282EVARALGKESLREVQubiquitination[4]
302AARDLVSKEGFRRARubiquitination[1, 2, 3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 422 AA 
Protein Sequence
MCAPPRGAPP EHPAPTGLCR SSCAELQARV MAALRQPQVA ELLAEARRAF REEFGAEPEL 60
AVSAPGRVNL IGEHTDYNQG LVLPMALELM TVLVGSPRKD GLVSLLTTSE GADEPQRLQF 120
PLPTAQRSLE PGTPRWANYV KGVIQYYPAA PLPGFSAVVV SSVPLGGGLS SSASLEVATY 180
TFLQQLCPDS GTIAARAQVC QQAEHSFAGM PCGIMDQFIS LMGQKGHALL IDCRSLETSL 240
VPLSDPKLAV LITNSNVRHS LASSEYPVRR RQCEEVARAL GKESLREVQL EELEAARDLV 300
SKEGFRRARH VVGEIRRTAQ AAAALRRGDY RAFGRLMVES HRSLRDDYEV SCPELDQLVE 360
AALAVPGVYG SRMTGGGFGG CTVTLLEASA APHAMRHIQE HYGGTATFYL SQAADGAKVL 420
CL 422 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004335; F:galactokinase activity; IEA:InterPro.
 GO:0006012; P:galactose metabolic process; IEA:InterPro. 
Interpro
 IPR000705; Galactokinase.
 IPR019741; Galactokinase_CS.
 IPR019539; GalKase_gal-bd.
 IPR013750; GHMP_kinase_C_dom.
 IPR006204; GHMP_kinase_N_dom.
 IPR006203; GHMP_knse_ATP-bd_CS.
 IPR006206; Mevalonate/galactokinase.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr. 
Pfam
 PF10509; GalKase_gal_bdg
 PF08544; GHMP_kinases_C
 PF00288; GHMP_kinases_N 
SMART
  
PROSITE
 PS00106; GALACTOKINASE
 PS00627; GHMP_KINASES_ATP 
PRINTS
 PR00473; GALCTOKINASE.
 PR00959; MEVGALKINASE.